Rat Fibulin 1 (FBLN1) ELISA Kit | FBLN1 elisa kit

Rat Fibulin 1 (FBLN1) ELISA Kit

Cat. Num. AAA2704478

• Gene Names: FBLN1; FBLN; FIBL1
• Reactivity: Rat
• Synonyms: Fibulin 1 (FBLN1); Rat Fibulin 1 (FBLN1) ELISA Kit; FBLN1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibulin 1 (FBLN1).; No significant cross-reactivity or interference between Fibulin 1 (FBLN1) and analogues was observed.
• Sequence Length: 683

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.141ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 1 (FBLN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 1 (FBLN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBLN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 1 (FBLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 1 (FBLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 1 (FBLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibulin 1 (FBLN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18490682
• NCBI GeneID: 2192
• Molecular Weight: 74,434 Da
• NCBI Official Full Name: Fibulin 1
• NCBI Official Synonym Full Names: fibulin 1
• NCBI Official Symbol: FBLN1
• NCBI Official Synonym Symbols: FBLN; FIBL1
• NCBI Protein Information: fibulin-1
• UniProt Protein Name: Fibulin-1
• UniProt Gene Name: FBLN1
• UniProt Synonym Gene Names: FIBL-1

NCBI Description

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]

Uniprot Description

Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.