Human ER Lipid Raft Associated Protein 1 (ERLIN1) RTU ELISA Kit | ERLIN1 rtu elisa kit

Human ER Lipid Raft Associated Protein 1 (ERLIN1) ELISA Kit

Cat. Num. AAA459112

• Gene Names: ERLIN1; KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69
• Reactivity: Human
• Synonyms: ER Lipid Raft Associated Protein 1 (ERLIN1); Human ER Lipid Raft Associated Protein 1 (ERLIN1) ELISA Kit; C10orf69; Erlin-1; KE04; KEO4; SPFH1; ER Lipid Raft Associated Protein 1; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1; ERLIN1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 348

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.107ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERLIN1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ERLIN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ERLIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ERLIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ERLIN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 154800489
• NCBI GeneID: 10613
• NCBI Accession #: NP_001094096.1
• NCBI GenBank Nucleotide #: NM_001100626.1
• UniProt Accession #: O75477; Q53HV0; B0QZ42
• Molecular Weight: 38,926 Da
• NCBI Official Full Name: erlin-1 isoform a
• NCBI Official Synonym Full Names: ER lipid raft associated 1
• NCBI Official Symbol: ERLIN1
• NCBI Official Synonym Symbols: KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69
• NCBI Protein Information: erlin-1
• UniProt Protein Name: Erlin-1
• Protein Family: Erlin
• UniProt Gene Name: ERLIN1
• UniProt Synonym Gene Names: C10orf69; KE04; KEO4; SPFH1; SPFH domain-containing protein 1

NCBI Description

The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62. [provided by RefSeq, Dec 2016]

Uniprot Description

Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. Binds cholesterol and may promote ER retention of the SCAP-SREBF complex (PubMed:24217618).