Human Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1) ELISA Kit | ERLIN1 elisa kit

Human Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1) ELISA Kit

Cat. Num. AAA2024380

• Gene Names: ERLIN1; KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69
• Reactivity: Human
• Synonyms: Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1); Human Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1) ELISA Kit; C10orf69; Erlin-1; KE04; KEO4; SPFH1; ER Lipid Raft Associated Protein 1; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1; ERLIN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1).; No significant cross-reactivity or interference between Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1) and analogues was observed.
• Sequence Length: 346

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.107ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERLIN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ERLIN1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 21618849
• NCBI GeneID: 10613
• UniProt Accession #: O75477; Q53HV0; B0QZ42
• Molecular Weight: 38,926 Da
• NCBI Official Full Name: ER lipid raft associated 1
• NCBI Official Synonym Full Names: ER lipid raft associated 1
• NCBI Official Symbol: ERLIN1
• NCBI Official Synonym Symbols: KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69
• NCBI Protein Information: erlin-1
• UniProt Protein Name: Erlin-1
• Protein Family: Erlin
• UniProt Gene Name: ERLIN1
• UniProt Synonym Gene Names: C10orf69; KE04; KEO4; SPFH1; SPFH domain-containing protein 1
• UniProt Entry Name: ERLN1_HUMAN

Uniprot Description

SPFH1: Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Belongs to the band 7/mec-2 family. Forms a heteromeric complex with ERLIN2.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q24.31

Cellular Component: endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding

Biological Process: ER-associated protein catabolic process