Human Erlin-1 (ERLIN1) ELISA Kit | Erlin-1 elisa kit

Human Erlin-1 (ERLIN1) ELISA Kit

Cat. Num. AAA7606223

• Gene Names: ERLIN1; KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69
• Reactivity: Human
• Synonyms: Erlin-1 (ERLIN1); Human Erlin-1 (ERLIN1) ELISA Kit; Erlin-1 elisa kit

• Reactivity: Human
• Sequence Length: 348

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Erlin-1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Erlin-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Erlin-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Erlin-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Erlin-1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 154800489
• NCBI GeneID: 10613
• NCBI Accession #: NP_001094096.1
• NCBI GenBank Nucleotide #: NM_001100626.1
• UniProt Accession #: O75477; Q53HV0; B0QZ42
• Molecular Weight: 38,926 Da
• NCBI Official Full Name: erlin-1 isoform a
• NCBI Official Synonym Full Names: ER lipid raft associated 1
• NCBI Official Symbol: ERLIN1
• NCBI Official Synonym Symbols: KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69
• NCBI Protein Information: erlin-1
• UniProt Protein Name: Erlin-1
• UniProt Gene Name: ERLIN1
• UniProt Synonym Gene Names: C10orf69; KE04; KEO4; SPFH1; SPFH domain-containing protein 1

NCBI Description

The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62. [provided by RefSeq, Dec 2016]

Uniprot Description

SPFH1: Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Belongs to the band 7/mec-2 family. Forms a heteromeric complex with ERLIN2.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q24.31

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; protein complex

Molecular Function: cholesterol binding; protein binding

Biological Process: ER-associated protein catabolic process; negative regulation of cholesterol biosynthetic process; negative regulation of fatty acid biosynthetic process; SREBP-mediated signaling pathway; transmembrane transport

Disease: Spastic Paraplegia 62, Autosomal Recessive