Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) RTU ELISA Kit | ENPP1 rtu elisa kit

Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit

Cat. Num. AAA459065

• Gene Names: ENPP1; M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
• Reactivity: Human
• Synonyms: Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1); Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit; M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1; Membrane component chromosome 6 surface marker 1; Alkaline phosphodiesterase I; Plasma-cell membrane glycoprotein PC-1; ENPP1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 925

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.062ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ENPP1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ENPP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ENPP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ENPP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ENPP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 170650661
• NCBI GeneID: 5167
• NCBI Accession #: NP_006199.2
• NCBI GenBank Nucleotide #: NM_006208.2
• UniProt Accession #: P22413; Q5T9R6; Q9NPZ3; Q9P1P6; Q9UP61; Q9Y6K3
• Molecular Weight: 104,924 Da
• NCBI Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 1
• NCBI Official Symbol: ENPP1
• NCBI Official Synonym Symbols: M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Protein Family: Ectonucleotide pyrophosphatase/phosphodiesterase family
• UniProt Gene Name: ENPP1
• UniProt Synonym Gene Names: M6S1; NPPS; PC1; PDNP1; NPPase

NCBI Description

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity and function.