Typical Testing Data/Standard Curve (for reference only)
Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA Kit | ENPP1 elisa kit
Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]
Uniprot Description
ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Protein type: EC 3.6.1.9; Phosphodiesterase; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - riboflavin; Carbohydrate Metabolism - starch and sucrose; Membrane protein, integral; EC 3.1.4.1; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6q22-q23
Cellular Component: basolateral plasma membrane; cell surface; extracellular space; integral to membrane; integral to plasma membrane; lysosomal membrane; plasma membrane
Molecular Function: 3'-phosphoadenosine 5'-phosphosulfate binding; ATP binding; calcium ion binding; insulin receptor binding; nucleic acid binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; protein binding; protein homodimerization activity; scavenger receptor activity; zinc ion binding
Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; biomineral formation; cellular phosphate ion homeostasis; cellular response to insulin stimulus; generation of precursor metabolites and energy; immune response; inorganic diphosphate transport; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; nucleoside triphosphate catabolic process; phosphate metabolic process; receptor-mediated endocytosis; regulation of bone mineralization; riboflavin metabolic process; sequestering of triacylglycerol; vitamin metabolic process; water-soluble vitamin metabolic process
Disease: Arterial Calcification, Generalized, Of Infancy, 1; Cole Disease; Diabetes Mellitus, Noninsulin-dependent; Hypophosphatemic Rickets, Autosomal Recessive, 2; Obesity
Research Articles on ENPP1
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Product Notes
The Human ENPP1 enpp1 (Catalog #AAA289596) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA289596 ELISA Kit recognizes Human ENPP1. It is sometimes possible for the material contained within the vial of "Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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