Mouse anti-Human, Mouse AHNAK1 Monoclonal Antibody | anti-AHNAK1 antibody

Anti-AHNAK1 Purified

Cat. Num. AAA1800594

• Gene Names: AHNAK; PM227; AHNAKRS
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: AHNAK1; Monoclonal Antibody; Anti-AHNAK1 Purified; AHNAK1 nucleoprotein; Desmoyokin; anti-AHNAK1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: EM-09
• Specificity: The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 5890

• Applicable Applications for anti-AHNAK1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Frozen, Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Immunogen: Bacterially expressed fragment of N-terminal domain of human AHNAK1.
• Preparation and Storage: Store at 2-8 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).)

Related Product Information for anti-AHNAK1 antibody

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

NCBI and Uniprot Product Information

• NCBI GI #: 61743954
• NCBI GeneID: 79026
• NCBI Accession #: NP_001611.1
• NCBI GenBank Nucleotide #: NP_001611.1
• UniProt Accession #: Q09666
• NCBI Official Full Name: neuroblast differentiation-associated protein AHNAK isoform 1
• NCBI Official Synonym Full Names: AHNAK nucleoprotein
• NCBI Official Symbol: AHNAK
• NCBI Official Synonym Symbols: PM227; AHNAKRS
• NCBI Protein Information: neuroblast differentiation-associated protein AHNAK
• UniProt Protein Name: Neuroblast differentiation-associated protein AHNAK
• UniProt Gene Name: AHNAK
• UniProt Synonym Gene Names: PM227

NCBI Description

The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene. [provided by RefSeq, Oct 2016]

Uniprot Description

May be required for neuronal cell differentiation.