Human Beta-defensin 129 ELISA Kit | DEFB129 elisa kit

Human Beta-defensin 129 ELISA Kit

Cat. Num. AAA762698

• Gene Names: DEFB129; DEFB29; hBD-29; DEFB-29; C20orf87; bA530N10.3
• Reactivity: Human
• Synonyms: Beta-defensin 129; Human Beta-defensin 129 ELISA Kit; DEFB129 (Beta-defensin 129)/Beta-defensin 29/DEFB-29/Defensin; beta 129; DEFB129 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DEFB129. No significant cross-reactivity or interference between DEFB129 and analogues was observed.
• Sequence Length: 183

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 7.8-500pg/ml
• Sensitivity: <4.688pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DEFB129 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DEFB129 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFB129 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DEFB129 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DEFB129 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DEFB129 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DEFB129 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 18250304
• NCBI GeneID: 140881
• NCBI Accession #: NP_543021.1
• NCBI GenBank Nucleotide #: NM_080831.3
• Molecular Weight: 20,299 Da
• NCBI Official Full Name: beta-defensin 129
• NCBI Official Synonym Full Names: defensin beta 129
• NCBI Official Symbol: DEFB129
• NCBI Official Synonym Symbols: DEFB29; hBD-29; DEFB-29; C20orf87; bA530N10.3
• NCBI Protein Information: beta-defensin 129
• UniProt Protein Name: Beta-defensin 129
• Protein Family: Beta-defensin
• UniProt Gene Name: DEFB129
• UniProt Synonym Gene Names: C20orf87; DEFB29; DEFB-29
• UniProt Entry Name: DB129_HUMAN

NCBI Description

Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 20p13. [provided by RefSeq, Nov 2014]

Uniprot Description

DEFB129: Has antibacterial activity (Potential). Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: cell surface; extracellular region

Biological Process: defense response to bacterium; innate immune response