Human Capping Protein Muscle Z Line Beta (CAPZb) RTU ELISA Kit | CAPZb rtu elisa kit

Human Capping Protein Muscle Z Line Beta (CAPZb) ELISA Kit

Cat. Num. AAA458112

• Gene Names: CAPZB; CAPB; CAPZ; CAPPB
• Reactivity: Human
• Synonyms: Capping Protein Muscle Z Line Beta (CAPZb); Human Capping Protein Muscle Z Line Beta (CAPZb) ELISA Kit; CAPZ; CAPB; CAPPB; F-Actin-Capping Protein Subunit Beta; CAPZb rtu elisa kit

• Reactivity: Human
• Sequence Length: 277

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.052ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAPZb rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAPZb. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CAPZb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CAPZb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CAPZb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 330864679
• NCBI GeneID: 832
• NCBI Accession #: NP_001193469.1
• NCBI GenBank Nucleotide #: NM_001206540.2
• UniProt Accession #: P47756; Q32Q68; Q5U0L4; Q8TB49; Q9NUC4
• Molecular Weight: – Da
• NCBI Official Full Name: F-actin-capping protein subunit beta isoform 2
• NCBI Official Synonym Full Names: capping actin protein of muscle Z-line subunit beta
• NCBI Official Symbol: CAPZB
• NCBI Official Synonym Symbols: CAPB; CAPZ; CAPPB
• NCBI Protein Information: F-actin-capping protein subunit beta
• UniProt Protein Name: F-actin-capping protein subunit beta
• Protein Family: F-actin-capping protein
• UniProt Gene Name: CAPZB

NCBI Description

This gene encodes the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells. A pseudogene of this gene is located on the long arm of chromosome 2. Multiple alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, Aug 2013]

Uniprot Description

F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization.