Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Signal recognition particle 19 kDa protein (SRP19) Standard Curve.)
Mouse Signal Recognition Particle 19 kDa Protein (SRP19) ELISA Kit | SRP19 elisa kit
Mouse Signal Recognition Particle 19 kDa Protein (SRP19) ELISA Kit
Gene Names
Srp19; 2310020D23Rik
Reactivity
Mouse
Synonyms
Signal Recognition Particle 19 kDa Protein (SRP19); Mouse Signal Recognition Particle 19 kDa Protein (SRP19) ELISA Kit; SRP19 elisa kit
Reactivity
Mouse
Specificity
Mouse Signal recognition particle 19 kDa protein (SRP19) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SRP19. No significant cross-reactivity or interference between Mouse SRP19 and analogues was observed.
Sequence Length
144
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Signal recognition particle 19 kDa protein (SRP19) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Signal recognition particle 19 kDa protein (SRP19) Standard Curve.)
Related Product Information for SRP19 elisa kit
Description: This Mouse Signal recognition particle 19 kDa protein (SRP19) ELISA Kit employs a two-site sandwich ELISA to quantitate SRP19 in samples. An antibody specific for SRP19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SRP19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRP19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRP19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Sequence analysis showed that SRP19 contains a very basic C-terminal domain of 7 lysine residues interrupted by 2 glycine residues. Northern blot analysis revealed expression of a 0.9 kb transcript in HeLa cells. SDS-PAGE analysis showed that SRP19 is expressed as a 19-kD protein, identical in size to canine SRP19. Functional analysis determined that the SRP19 protein binds to 7SL RNA in canine pancreas.
Background: The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Sequence analysis showed that SRP19 contains a very basic C-terminal domain of 7 lysine residues interrupted by 2 glycine residues. Northern blot analysis revealed expression of a 0.9 kb transcript in HeLa cells. SDS-PAGE analysis showed that SRP19 is expressed as a 19-kD protein, identical in size to canine SRP19. Functional analysis determined that the SRP19 protein binds to 7SL RNA in canine pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Signal recognition particle 19 kDa protein
NCBI Official Synonym Full Names
signal recognition particle 19
NCBI Official Symbol
Srp19
NCBI Official Synonym Symbols
2310020D23Rik
NCBI Protein Information
signal recognition particle 19 kDa protein
UniProt Protein Name
Signal recognition particle 19 kDa protein
Protein Family
UniProt Gene Name
Srp19
UniProt Synonym Gene Names
SRP19
UniProt Entry Name
SRP19_MOUSE
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Product Notes
The Mouse SRP19 srp19 (Catalog #AAA9716869) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716869 ELISA Kit recognizes Mouse SRP19. It is sometimes possible for the material contained within the vial of "Signal Recognition Particle 19 kDa Protein (SRP19), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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