Human Bone Morphogenetic Protein Receptor 1B (BMPR1B) RTU ELISA Kit | BMPR1B rtu elisa kit

Human Bone Morphogenetic Protein Receptor 1B (BMPR1B) ELISA Kit

Cat. Num. AAA457968

• Gene Names: BMPR1B; ALK6; AMDD; BDA2; ALK-6; BDA1D; CDw293
• Reactivity: Human
• Synonyms: Bone Morphogenetic Protein Receptor 1B (BMPR1B); Human Bone Morphogenetic Protein Receptor 1B (BMPR1B) ELISA Kit; CDw293; CD293; BMPR1-B; ALK6; BMPR1B rtu elisa kit

• Reactivity: Human
• Sequence Length: 502

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.134ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMPR1B rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMPR1B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BMPR1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMPR1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BMPR1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502431
• NCBI GeneID: 658
• NCBI Accession #: NP_001194.1
• NCBI GenBank Nucleotide #: NM_001203.2
• UniProt Accession #: O00238; P78366; B2R953; B4DSV1
• Molecular Weight: 60,582 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-1B isoform b
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor type 1B
• NCBI Official Symbol: BMPR1B
• NCBI Official Synonym Symbols: ALK6; AMDD; BDA2; ALK-6; BDA1D; CDw293
• NCBI Protein Information: bone morphogenetic protein receptor type-1B
• UniProt Protein Name: Bone morphogenetic protein receptor type-1B
• Protein Family: Bone morphogenetic protein receptor
• UniProt Gene Name: BMPR1B
• UniProt Synonym Gene Names: BMP type-1B receptor; BMPR-1B

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. Positively regulates chondrocyte differentiation through GDF5 interaction.