Goat CHRNA7 Polyclonal Antibody | anti-CHRNA7 antibody

Goat Polyclonal to Human CHRNA7

Cat. Num. AAA246718

• Gene Names: CHRNA7; NACHRA7; CHRNA7-2
• Reactivity: Bovine, Chicken, Dog, Horse, Human, Monkey, Pig
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immnunizing peptide.
• Synonyms: CHRNA7; Polyclonal Antibody; Goat Polyclonal to Human CHRNA7; Anti-CHRNA7 Antibody IHC-plus; AChR-alpha7; Acra 7; CHRNA7-2; NAChR alpha 7; NACHRA7; Human CHRNA7; anti-CHRNA7 antibody

• Host: Goat
• Reactivity: Bovine, Chicken, Dog, Horse, Human, Monkey, Pig
• Clonality: Polyclonal
• Specificity: Human CHRNA7. This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immnunizing peptide.
• Form/Format: TBS, pH7.3, 0.02% Sodium Azide, 0.5%BSA
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 502

• Applicable Applications for anti-CHRNA7 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA, IHC-P (3 - 6 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Immunohistochemistry: (MBS246718) was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH...
• Target: CHRNA7
• Immunogen: CHRNA7 antibody was raised against peptide with sequence KRPGEDKVRPACQHKQ from an internal region of the protein sequence according to (NP_000737.1).
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliqout and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CHRNA7 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

Product Categories/Family for anti-CHRNA7 antibody

Family: Ion Channel; Subfamily: Nicotinic acetylcholine receptor

NCBI and Uniprot Product Information

• NCBI GI #: 4502831
• NCBI GeneID: 1139
• NCBI Accession #: NP_000737.1
• NCBI GenBank Nucleotide #: NM_000746.5
• UniProt Accession #: P36544; Q15826; Q8IUZ4; Q96RH2; Q99555; Q9BXH0; A8K7Q4; B4DFS0
• Molecular Weight: 35,482 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-7 isoform 1
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 7 (neuronal)
• NCBI Official Symbol: CHRNA7
• NCBI Official Synonym Symbols: NACHRA7; CHRNA7-2
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-7; a7 nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha 7 neuronal nicotinic acetylcholine receptor; cholinergic receptor, nicotinic, alpha polypeptide 7; neuronal acetylcholine receptor protein, alpha-7 chain
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-7
• Protein Family: CHRNA7-FAM7A fusion protein
• UniProt Gene Name: CHRNA7
• UniProt Synonym Gene Names: NACHRA7
• UniProt Entry Name: ACHA7_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

nAChRA7: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 7/CHRNA7 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Channel, cation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: toxin binding; chloride channel regulator activity; protein homodimerization activity; beta-amyloid binding; acetylcholine-gated cation channel activity; acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding

Biological Process: response to nicotine; activation of MAPK activity; negative regulation of tumor necrosis factor production; signal transduction; memory; cellular calcium ion homeostasis; synaptic transmission; positive regulation of angiogenesis; calcium ion transport; positive regulation of cell proliferation; response to hypoxia; ion transport; cognition

Disease: Chromosome 15q13.3 Deletion Syndrome