Human ATP Binding Cassette Transporter G2 (ABCG2) RTU ELISA Kit | ABCG2 rtu elisa kit

Human ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit

Cat. Num. AAA457309

• Gene Names: ABCG2; MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
• Reactivity: Human
• Synonyms: ATP Binding Cassette Transporter G2 (ABCG2); Human ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit; CDw338; CD338; ABC-G2; MRX; BCRP1; ABC15; ABCP; BCRP; BMDP; MXR; MXR1; Mitoxantrone resistance-associated protein; Breast Cancer Resistance Protein 1; Urate exporter; ABCG2 rtu elisa kit

• Reactivity: Human
• Sequence Length: 611

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCG2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ABCG2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ABCG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ABCG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ABCG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 383792176
• NCBI GeneID: 9429
• NCBI Accession #: NP_001244315.1
• NCBI GenBank Nucleotide #: NM_001257386.2
• UniProt Accession #: Q9UNQ0; O95374; Q4W5I3; Q53ZQ1; Q569L4; Q5YLG4; Q86V64; Q8IX16; Q96LD6; Q96TA8; A0A1W3; A8K1T5
• Molecular Weight: 67,453 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 2 isoform 2
• NCBI Official Synonym Full Names: ATP binding cassette subfamily G member 2 (Junior blood group)
• NCBI Official Symbol: ABCG2
• NCBI Official Synonym Symbols: MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
• NCBI Protein Information: ATP-binding cassette sub-family G member 2
• UniProt Protein Name: ATP-binding cassette sub-family G member 2
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG2
• UniProt Synonym Gene Names: ABCP; BCRP; BCRP1; MXR

NCBI Description

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. Implicated in the efflux of numerous drugs and xenobiotics: mitoxantrone, the photosensitizer pheophorbide, camptothecin, methotrexate, azidothymidine (AZT), and the anthracyclines daunorubicin and doxorubicin.