Human Aminolevulinate Delta Dehydratase (ALAD) RTU ELISA Kit | ALAD rtu elisa kit

Human Aminolevulinate Delta Dehydratase (ALAD) ELISA Kit

Cat. Num. AAA457516

• Gene Names: ALAD; PBGS; ALADH
• Reactivity: Human
• Synonyms: Aminolevulinate Delta Dehydratase (ALAD); Human Aminolevulinate Delta Dehydratase (ALAD) ELISA Kit; ALADH; PBGS; Delta-Aminolevulinic Acid Dehydratase; Porphobilinogen Synthase; ALAD rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ALAD. No significant cross-reactivity or interference between ALAD and analogues was observed.
• Sequence Length: 330

• Samples: Human Serum, Erythrocyte Lysates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.128ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALAD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALAD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALAD rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ALAD in human serum, erythrocyte lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALAD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ALAD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 189083849
• NCBI GeneID: 210
• NCBI Accession #: NP_000022.3
• NCBI GenBank Nucleotide #: NM_000031.5
• UniProt Accession #: P13716; Q16870; Q16871; Q9BVQ9; A8K375; B2R6F2
• Molecular Weight: 39,034 Da
• NCBI Official Full Name: delta-aminolevulinic acid dehydratase isoform b
• NCBI Official Synonym Full Names: aminolevulinate dehydratase
• NCBI Official Symbol: ALAD
• NCBI Official Synonym Symbols: PBGS; ALADH
• NCBI Protein Information: delta-aminolevulinic acid dehydratase
• UniProt Protein Name: Delta-aminolevulinic acid dehydratase
• UniProt Gene Name: ALAD
• UniProt Synonym Gene Names: ALADH

NCBI Description

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.