Aminolevulinate Delta Dehydratase (ALAD) ELISA Kit | ALAD elisa kit

Rat Aminolevulinate Delta Dehydratase (ALAD) ELISA Kit

Cat. Num. AAA2704336

• Gene Names: ALAD; PBGS; ALADH
• Reactivity: Rat
• Synonyms: Aminolevulinate Delta Dehydratase (ALAD); Rat Aminolevulinate Delta Dehydratase (ALAD) ELISA Kit; ALADH; PBGS; Delta-Aminolevulinic Acid Dehydratase; Porphobilinogen Synthase; ALAD elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aminolevulinate Delta Dehydratase (ALAD).; No significant cross-reactivity or interference between Aminolevulinate Delta Dehydratase (ALAD) and analogues was observed.
• Sequence Length: 359

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.066ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aminolevulinate Delta Dehydratase (ALAD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aminolevulinate Delta Dehydratase (ALAD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALAD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminolevulinate Delta Dehydratase (ALAD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminolevulinate Delta Dehydratase (ALAD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminolevulinate Delta Dehydratase (ALAD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aminolevulinate Delta Dehydratase (ALAD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119607786
• NCBI GeneID: 210
• UniProt Accession #: P13716; Q16870; Q16871; Q9BVQ9; A8K375; B2R6F2
• Molecular Weight: 39,034 Da
• NCBI Official Full Name: aminolevulinate, delta-, dehydratase, isoform CRA_a
• NCBI Official Synonym Full Names: aminolevulinate dehydratase
• NCBI Official Symbol: ALAD
• NCBI Official Synonym Symbols: PBGS; ALADH
• NCBI Protein Information: delta-aminolevulinic acid dehydratase
• UniProt Protein Name: Delta-aminolevulinic acid dehydratase
• UniProt Gene Name: ALAD
• UniProt Synonym Gene Names: ALADH

NCBI Description

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.