Typical Testing Data/Standard Curve (for reference only)
Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) CLIA Kit | PACAP clia kit
Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PACAP. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PACAP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PACAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PACAP. You can calculate the concentration of PACAP in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
Uniprot Description
ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 18p11
Cellular Component: cytosol; extracellular region; extracellular space; intracellular; terminal button
Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; pituitary adenylate cyclase activating polypeptide activity; pituitary adenylate cyclase-activating peptide receptor binding; protein binding; receptor binding; receptor signaling protein activity
Biological Process: adenylate cyclase activation; ATP metabolic process; behavioral fear response; cAMP-mediated signaling; cell-cell signaling; elevation of cytosolic calcium ion concentration; female pregnancy; histamine secretion; negative regulation of acute inflammatory response to antigenic stimulus; negative regulation of acute inflammatory response to non-antigenic stimulus; negative regulation of cell cycle; negative regulation of potassium ion transport; nerve growth factor receptor signaling pathway; neurite development; neuropeptide signaling pathway; ovarian follicle development; pituitary gland development; positive regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of GTPase activity; positive regulation of interleukin-6 production; positive regulation of protein kinase activity; positive regulation of synaptic transmission, glutamatergic; positive regulation of transcription from RNA polymerase II promoter; positive regulation of vasodilation; regulation of G-protein coupled receptor protein signaling pathway; regulation of postsynaptic membrane potential; regulation of protein localization; response to ethanol; response to starvation; sensory perception of pain; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on PACAP
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Product Notes
The Mouse PACAP adcyap1 (Catalog #AAA2533083) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533083 CLIA Kit recognizes Mouse PACAP. It is sometimes possible for the material contained within the vial of "PACAP (Pituitary Adenylate Cyclase Activating Polypeptide), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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