Mouse Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14) ELISA Kit | BCL2L14 elisa kit

Mouse Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14) ELISA Kit

Cat. Num. AAA7246329

• Gene Names: BCL2L14; BCLG
• Reactivity: Mouse
• Synonyms: Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14); Mouse Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14) ELISA Kit; BCL2L14 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BCL2L14 elisa kit

Intended Uses: This BCL2L14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BCL2L14. This ELISA kit for research use only!

Principle of the Assay: BCL2L14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BCL2L14 antibody and an BCL2L14-HRP conjugate. The assay sample and buffer are incubated together with BCL2L14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2L14 concentration since BCL2L14 from samples and BCL2L14-HRP conjugate compete for the anti-BCL2L14 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2L14 from the sample, fewer sites are left to bind BCL2L14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL2L14 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BCL2L14 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 21040328
• NCBI GeneID: 79370
• NCBI Accession #: NP_620048.1
• NCBI GenBank Nucleotide #: NM_138722.1
• Molecular Weight: 30,948 Da
• NCBI Official Full Name: apoptosis facilitator Bcl-2-like protein 14 isoform 1
• NCBI Official Synonym Full Names: BCL2-like 14 (apoptosis facilitator)
• NCBI Official Symbol: BCL2L14
• NCBI Official Synonym Symbols: BCLG
• NCBI Protein Information: apoptosis facilitator Bcl-2-like protein 14; bcl2-L-14; apoptosis regulator BCL-G
• UniProt Protein Name: Apoptosis facilitator Bcl-2-like protein 14
• Protein Family: Apoptosis facilitator Bcl-2-like protein
• UniProt Gene Name: BCL2L14
• UniProt Synonym Gene Names: BCLG; Bcl2-L-14
• UniProt Entry Name: B2L14_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of this gene has been shown to induce apoptosis in cells. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported for this gene. [provided by RefSeq, May 2009]

Uniprot Description

Bcl-G: Plays a role in apoptosis. Belongs to the Bcl-2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12p13-p12

Cellular Component: membrane; cytoplasm; intracellular organelle; endomembrane system; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: regulation of apoptosis; apoptosis