ELISA (EIA)
( Binding curve of anti-EGFR antibody Matuzumab to EGFR-Fc fusion protein ELISA Plate coated with human EGFR-Fc (Pr00117-10.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.)
EGFR recombinant antibody
Anti-EGFR [Matuzumab]
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Protein A Affinity Purified
Synonyms
EGFR; Anti-EGFR [Matuzumab]; Epidermal Growth Factor Receptor; mAb425; EGFR recombinant antibody
Reactivity
Human
Isotype
Human IgG1, kappa
Specificity
Binds to human EGFR.
Purity/Purification
Protein A Affinity Purified
Sequence Length
10
Applicable Applications for EGFR recombinant antibody
Block, Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
This antibody binds to human EGFR at a side distinct frm the antigen binding site, preventing conformational re-arrangement required for dimerization, a process essential for high-affinitiy ligand binding (Schmiedel 2008).
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
Domain III of the extracellular domain of human EGRF.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
ELISA (EIA)
( Binding curve of anti-EGFR antibody Matuzumab to EGFR-Fc fusion protein ELISA Plate coated with human EGFR-Fc (Pr00117-10.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.)
ELISA (EIA)
( Binding curve of anti-EGFR antibody Matuzumab to EGFR-Fc fusion protein ELISA Plate coated with human EGFR-Fc (Pr00117-10.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of mouse skin tissue using anti-EGFR antibody Matuzumab. Anti-EGFR staining of formaldehyde fixed paraffin embedded mouse skin tissue, at 40x magnification. The human IgG1-chimeric version of Matuzumab was used to stain samples at a concentration of 5 ug/ml.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of mouse skin tissue using anti-EGFR antibody Matuzumab. Anti-EGFR staining of formaldehyde fixed paraffin embedded mouse skin tissue, at 40x magnification. The human IgG1-chimeric version of Matuzumab was used to stain samples at a concentration of 5 ug/ml.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of rat stomach tissue using anti-EGFR antibody Matuzumab. Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of Matuzumab was used to stain samples at a concentration of 5 ug/ml.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of rat stomach tissue using anti-EGFR antibody Matuzumab. Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of Matuzumab was used to stain samples at a concentration of 5 ug/ml.)
Related Product Information for EGFR recombinant antibody
Recombinant monoclonal antibody to EGFR. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody Matuzumab.
Product Categories/Family for EGFR recombinant antibody
References
Murthy et al. Binding of an antagonistic monoclonal antibody to an intact and fragmented EGF-receptor polypeptide. Arch Biochem Biophys. 1987 Feb 1; 252(2):549-60. PMID:2434025
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
epidermal growth factor receptor, partial
Protein Family
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Product Notes
The EGFR (Catalog #AAA488208) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-EGFR [Matuzumab] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, Block, Flow Cytometry (FC/FACS), Western Blot (WB). This antibody binds to human EGFR at a side distinct frm the antigen binding site, preventing conformational re-arrangement required for dimerization, a process essential for high-affinitiy ligand binding (Schmiedel 2008). Researchers should empirically determine the suitability of the EGFR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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