BD1 Protein | DEFB1 protein
BD1, Human (beta Defensin-1) (Control Peptide)
Gene Names
DEFB1; BD1; HBD1; DEFB-1; DEFB101
Applications
ELISA
Purity
Highly PurifiedHighly purified
Synonyms
BD1; Human (beta Defensin-1) (Control Peptide); DEFB1 protein
Host
Human
Specificity
Synthetic peptide (17aa) near the N-terminus of mature human defensin-1. No significant homology exists with other beta-defensins (2-4). Species Sequence Homology
Purity/Purification
Highly Purified
Highly purified
Highly purified
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Applicable Applications for DEFB1 protein
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight.
Dilution: ELISA: Coat ELISA plates at 1ug/ml.
Antibody Blocking: 5-10ug Control Peptide per 1ul B0900-25 (antiserum) or 1ug B0900-30 (Affinity Purified antibody).
Dilution: ELISA: Coat ELISA plates at 1ug/ml.
Antibody Blocking: 5-10ug Control Peptide per 1ul B0900-25 (antiserum) or 1ug B0900-30 (Affinity Purified antibody).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for DEFB1 protein
Control peptide for B0900-25 (antiserum) and B0900-30 (Affinity Purified antibody).
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides. They were originally identified in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha- and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified. Alpha-defensins are encoded by genes designated DEFA1-6. Human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils. HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein. The HNPs are 29-30aa long and are identical in sequence except at the N-terminal amino acid. DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively. DEFA2 is identical to both except that it has 29 instead of 30 amino acids. Human defensin-1 is produced from 68aa precursor (mature peptide 33-69aa) peptide. Mouse defensin-1 is produced from 69aa precursor (mature peptide 33-69aa).
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides. They were originally identified in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha- and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified. Alpha-defensins are encoded by genes designated DEFA1-6. Human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils. HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein. The HNPs are 29-30aa long and are identical in sequence except at the N-terminal amino acid. DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively. DEFA2 is identical to both except that it has 29 instead of 30 amino acids. Human defensin-1 is produced from 68aa precursor (mature peptide 33-69aa) peptide. Mouse defensin-1 is produced from 69aa precursor (mature peptide 33-69aa).
Product Categories/Family for DEFB1 protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,420 Da
NCBI Official Full Name
beta-defensin 1 preproprotein
NCBI Official Synonym Full Names
defensin, beta 1
NCBI Official Symbol
DEFB1
NCBI Official Synonym Symbols
BD1; HBD1; DEFB-1; DEFB101
NCBI Protein Information
beta-defensin 1; BD-1; beta-defensin-1
UniProt Protein Name
Beta-defensin 1
Protein Family
UniProt Gene Name
DEFB1
UniProt Synonym Gene Names
BD1; HBD1; BD-1; hBD-1
UniProt Entry Name
DEFB1_HUMAN
NCBI Description
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]
Uniprot Description
defensin, beta 1: Has bactericidal activity. Belongs to the beta-defensin family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: extracellular space; Golgi lumen; extracellular region
Biological Process: G-protein coupled receptor protein signaling pathway; defense response to Gram-positive bacterium; innate immune response in mucosa; response to bacterium; antibacterial humoral response; innate immune response; immune response; response to testosterone stimulus; chemotaxis; acute inflammatory response
Research Articles on DEFB1
Similar Products
Product Notes
The DEFB1 defb1 (Catalog #AAA635035) is a Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Dilution: ELISA: Coat ELISA plates at 1ug/ml. Antibody Blocking: 5-10ug Control Peptide per 1ul B0900-25 (antiserum) or 1ug B0900-30 (Affinity Purified antibody). Researchers should empirically determine the suitability of the DEFB1 defb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BD1, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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