Beta-Defensin 1 (DEFB1) Recombinant Protein | DEFB1 recombinant protein

Beta-Defensin 1 (DEFB1)

Cat. Num. AAA516633

• Gene Names: DEFB1; BD1; HBD1; DEFB-1; DEFB101
• Applications: Cell Assay
• Synonyms: Beta-Defensin 1 (DEFB1); Defensin; beta 1; DEFB1; BD-1; hBD-1.; DEFB1 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS (pH8)

• Applicable Applications for DEFB1 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: The ED50 was determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1.0 ug/mL)

SDS-Page

(Purity: Recombinant Human Beta-Defensin 1 (DEFB1) resolved on a 17% SDS-PAGE gel under reducing conditions, and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for DEFB1 recombinant protein

Recombinant Human Beta-Defensin 1 (DEFB1) was expressed in E Coli cells. The protein accession number is P60022
Defensins are a large family of peptides of which two groups exist in mammals: alpha defensins and beta defensins -which are distinguishable by the spacing and connectivity of the conserved cysteine residues within the mature peptides. It is thought that the function of defensins in the eradication of pathogens from the host system is to insert themselves into the bacterial membrane under the influence of membrane potential, in doing so form channels which lead to leakage of cytoplasmic molecules and cell death. Human beta-defensin-1, which has 36 amino acids, was originally isolated from hemofiltrates of patients with advanced renal failure. The BD-1 gene is expressed mainly in the urogenital tract and, to a lesser degree, in trachea and lung, BD-1 is believed to function in the antimicrobial defense of the urogenital and respiratory tracts.

Product Categories/Family for DEFB1 recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4885181
• NCBI GeneID: 1672
• NCBI Accession #: NP_005209.1
• NCBI GenBank Nucleotide #: NM_005218.3
• UniProt Accession #: P60022; Q09753
• Molecular Weight: 7,420 Da
• NCBI Official Full Name: beta-defensin 1 preproprotein
• NCBI Official Synonym Full Names: defensin, beta 1
• NCBI Official Symbol: DEFB1
• NCBI Official Synonym Symbols: BD1; HBD1; DEFB-1; DEFB101
• NCBI Protein Information: beta-defensin 1; BD-1; beta defensin 1; beta-defensin-1
• UniProt Protein Name: Beta-defensin 1
• Protein Family: Beta-defensin
• UniProt Gene Name: DEFB1
• UniProt Synonym Gene Names: BD1; HBD1; BD-1; hBD-1
• UniProt Entry Name: DEFB1_HUMAN

NCBI Description

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]

Uniprot Description

defensin, beta 1: Has bactericidal activity. Belongs to the beta-defensin family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; Golgi lumen; extracellular region

Biological Process: G-protein coupled receptor protein signaling pathway; defense response to Gram-positive bacterium; innate immune response in mucosa; response to bacterium; antibacterial humoral response; innate immune response; immune response; response to testosterone stimulus; chemotaxis; acute inflammatory response