CSK elisa kit

Csk (Phospho-Ser364) Phospho Sandwich ELISA Kit

Cat. Num. AAA9511020

• Reactivity: Human, Mouse, Rat
• Synonyms: Csk; Csk (Phospho-Ser364) Phospho Sandwich ELISA Kit; C-SRC kinase; Protein-tyrosine kinase CYL; CSK elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The Csk (Phospho-Ser364) Phospho Sandwich ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can detect Csk protein phosphorylation and expression profile in cell lysates. The kit can be used for measuring the relative amounts of phosphorylated Csk in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Csk phosphorylation.
• Sequence Length: 450

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for ~6 months

Testing Data

(HeLa cells were grown to 90% confluency and were stimulated with PMA 150 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Csk2 P-Ser364 versus untreated HeLa lysates.)

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-Csk (Phospho-Ser364) Antibody.)

Related Product Information for CSK elisa kit

Principle of the Assay: The Phospho Sandwich Csk (Phospho-Ser364) ELISA Kit contains the components necessary for semi-quantitative determination of Csk (Phospho-Ser364) concentrations within experimental cell lysate samples. This particular immunoassay utilizes the traditional "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope Csk (Phospho-Ser364) while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer where data analysis may begin.

Product Categories/Family for CSK elisa kit

Phospho Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 187475373
• NCBI GeneID: 1445
• NCBI Accession #: NP_001120662.1
• NCBI GenBank Nucleotide #: NP_001120662.1
• UniProt Accession #: P41240
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: C-terminal Src kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK
• UniProt Entry Name: CSK_HUMAN

NCBI Description

The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; negative regulation of kinase activity; negative regulation of phagocytosis; cell migration; central nervous system development; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cytokine production; regulation of cell proliferation; positive regulation of MAP kinase activity; negative regulation of cell proliferation; T cell costimulation; negative regulation of interleukin-6 production; innate immune response; oligodendrocyte differentiation; morphogenesis of an epithelium; brain development; negative regulation of bone resorption; cell differentiation; blood coagulation; transmembrane receptor protein tyrosine kinase signaling pathway