Ezrin ELISA Kit | EZR elisa kit

Ezrin (Phospho-Thr566) Phospho Sandwich ELISA Kit

Cat. Num. AAA9511029

• Gene Names: EZR; CVL; CVIL; VIL2; HEL-S-105
• Reactivity: Human, Mouse, Rat
• Synonyms: Ezrin; Ezrin (Phospho-Thr566) Phospho Sandwich ELISA Kit; Cytovillin; EZRI; p81; VIL2; Villin 2; EZR elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The Ezrin (Phospho-Thr566) Phospho Sandwich ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can detect Ezrin protein phosphorylation and expression profile in cell lysates. The kit can be used for measuring the relative amounts of phosphorylated Ezrin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Ezrin phosphorylation.
• Sequence Length: 586

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for ~6 months

Testing Data

(HeLa cells were grown to 90% confluency and stimulated with 30 ng/mL TNF-)

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-Ezrin (Phospho-Thr566) Antibody.)

Related Product Information for EZR elisa kit

Principle of the Assay: The Phospho Sandwich Ezrin (Phospho-Thr566) ELISA Kit contains the components necessary for semi-quantitative determination of Ezrin (Phospho-Thr566) concentrations within experimental cell lysate samples. This particular immunoassay utilizes the traditional "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope Ezrin (Phospho-Thr566) while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer where data analysis may begin.

Product Categories/Family for EZR elisa kit

Phospho Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 161702986
• NCBI GeneID: 7430
• NCBI Accession #: NP_001104547.1
• NCBI GenBank Nucleotide #: NP_001104547.1
• UniProt Accession #: P15311
• NCBI Official Full Name: ezrin
• NCBI Official Synonym Full Names: ezrin
• NCBI Official Symbol: EZR
• NCBI Official Synonym Symbols: CVL; CVIL; VIL2; HEL-S-105
• NCBI Protein Information: ezrin
• UniProt Protein Name: Ezrin
• Protein Family: Ezrin
• UniProt Gene Name: EZR
• UniProt Synonym Gene Names: VIL2
• UniProt Entry Name: EZRI_HUMAN

NCBI Description

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: cortical cytoskeleton; extracellular space; focal adhesion; microvillus; basolateral plasma membrane; T-tubule; actin filament; cytosol; actin cytoskeleton; lipid raft; ruffle; extrinsic to membrane; membrane; microvillus membrane; apical part of cell; apical plasma membrane; plasma membrane; nucleolus; uropod; vesicle; filopodium

Molecular Function: actin filament binding; protein domain specific binding; protein binding; cell adhesion molecule binding

Biological Process: microvillus biogenesis; axon guidance; actin filament bundle formation; regulation of cell shape; leukocyte adhesion; filopodium formation; cytoskeletal anchoring; receptor internalization; membrane to membrane docking; establishment of epithelial cell polarity