Human Sjogren Syndrome Antigen B ELISA Kit | SSB elisa kit

Human SSB (Sjogren Syndrome Antigen B) ELISA Kit

Cat. Num. AAA2533454

• Gene Names: SSB; La; LARP3; La/SSB
• Reactivity: Human
• Synonyms: Sjogren Syndrome Antigen B; Human SSB (Sjogren Syndrome Antigen B) ELISA Kit; SSB elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human SSB in samples. No significant cross-reactivity or interference between Human SSB and analogues was observed.
• Sequence Length: 48

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SSB were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SSB were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SSB elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SSB concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SSB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SSB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SSB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SSB. You can calculate the concentration of Human SSB in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 86651712
• NCBI GeneID: 6741
• Molecular Weight: 46,837 Da
• NCBI Official Full Name: Sjogren syndrome antigen B, partial
• NCBI Official Synonym Full Names: Sjogren syndrome antigen B
• NCBI Official Symbol: SSB
• NCBI Official Synonym Symbols: La; LARP3; La/SSB
• NCBI Protein Information: lupus La protein
• UniProt Protein Name: Lupus La protein
• Protein Family: Single-stranded DNA binding protein
• UniProt Gene Name: SSB
• UniProt Synonym Gene Names: SS-B
• UniProt Entry Name: LA_HUMAN

NCBI Description

The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]

Uniprot Description

SSB: a nuclear protein that plays a role in the transcription of RNA polymerase III. It is most probably a transcription termination factor. Binds to the 3' termini of virtually all nascent polymerase III transcripts. It is associated with precursor forms of RNA polymerase III transcripts including tRNA and 4.5S, 5s, 7s, and 7-2 RNAs.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: nuclear chromosome, telomeric region; ribonucleoprotein complex

Molecular Function: mRNA binding; nucleotide binding; protein binding; tRNA binding

Biological Process: histone mRNA metabolic process; tRNA modification