PLB Antibody | anti-PLB antibody

PLB (Phospho-Ser16+Thr17) Antibody

Cat. Num. AAA858760

• Gene Names: PLN; PLB; CMD1P; CMH18
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: PLB; PLB (Phospho-Ser16+Thr17) Antibody; PLN; PPLA; PPLA_DOG; cardiac phospholamban; phospholamban; anti-PLB antibody

• Reactivity: Human, Mouse, Rat
• Specificity: PLB (Phospho-Ser16+Thr17) antibody detects endogenous levels of PLB only when phosphorylated at serine 16 and threonine 17.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 52

• Applicable Applications for anti-PLB antibody: Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IF: 1:500-1:1000; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PLB around the phosphorylation site of serine 16 and threonine 17 (R-A-S^P-T^P-I-E).
• All Sites: Human: Ser16+Thr17; Mouse: Ser16+Thr17; Rat: Ser16+Thr17
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HuvEc cells, using PLB (Phospho-Ser16+Thr17) antibody.)

Product Categories/Family for anti-PLB antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Fujii J., J. Biol. Chem. 266:11669-11675(1991). McTiernan C.F., J. Mol. Cell. Cardiol. 31:679-692(1999). Haghighi K., Proc. Natl. Acad. Sci. U.S.A. 103:1388-1393(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4505887
• NCBI GeneID: 5350
• NCBI Accession #: NP_002658.1
• NCBI GenBank Nucleotide #: NM_002667.4
• UniProt Accession #: P26678
• NCBI Official Full Name: cardiac phospholamban
• NCBI Official Synonym Full Names: phospholamban
• NCBI Official Symbol: PLN
• NCBI Official Synonym Symbols: PLB; CMD1P; CMH18
• NCBI Protein Information: cardiac phospholamban
• UniProt Protein Name: Cardiac phospholamban
• Protein Family: Lysophospholipase
• UniProt Gene Name: PLN
• UniProt Synonym Gene Names: PLB; PLB

NCBI Description

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016]

Uniprot Description

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.