SDS-Page
Trypsin Recombinant Protein
Recombinant Pig Trypsin
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Trypsin; Recombinant Pig Trypsin; Trypsin recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
9-231aa; Full Length of Mature Protein
Sequence
IVGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNIDVLEGNEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCAAAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGFLEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVN
WIQQTIAAN
Production Note
Special Offer: The Yeast host-expressed protein is manufactured from a stock plasmid containing the protein gene. Yeasthost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The Yeast host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select Yeast host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-Page
SDS-Page
References
On trypsinogen and trypsin of pig.Charles M., Rovery M., Guidoni A.A., Desnuelle P.Biochim. Biophys. Acta 69:115-129(1963)
Determination of the amino acid sequence of porcine trypsin by sequenator analysis.Hermodson M.A., Ericsson L.H., Neurath H., Walsh K.A.Biochemistry 12:3146-3153(1973)
Refined 1.6-A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex.Huang Q., Liu S., Tang Y.J. Mol. Biol. 229:1022-1036(1993)
Amino acid sequencing of a trypsin inhibitor by refined 1.6 A X-ray crystal structure of its complex with porcine beta-trypsin.Huang Q., Liu S., Tang Y., Zeng F., Qian R.FEBS Lett. 297:143-146(1992)
Refined 1.8-A resolution crystal structure of the porcine epsilon-trypsin.Huang Q., Wang Z., Li Y., Liu S., Tang Y.Biochim. Biophys. Acta 1209:77-82(1994)
The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition.Stubbs M.T., Morenweiser R., Stuerzebecher J., Bauer M., Bode W., Huber R., Piechottka G.P., Matschiner G., Sommerhoff C.P., Fritz H., Auerswald E.A.J. Biol. Chem. 272:19931-19937(1997)
Structure of the complex of leech-derived tryptase inhibitor (LDTI)
with trypsin and modeling of the LDTI-tryptase system.di Marco S., Priestle J.P.Structure 5:1465-1474(1997)
L-isoaspartate 115 of porcine beta-trypsin promotes crystallization of its complex with bdellastasin.Rester U., Moser M., Huber R., Bode W.Acta Crystallogr. D 56:581-588(2000)
NCBI and Uniprot Product Information
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Product Notes
The Trypsin (Catalog #AAA969599) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 9-231aa; Full Length of Mature Protein. The amino acid sequence is listed below: IVGGYTCAAN SIPYQVSLNS GSHFCGGSLI NSQWVVSAAH CYKSRIQVRL GEHNIDVLEG NEQFINAAKI ITHPNFNGNT LDNDIMLIKL SSPATLNSRV ATVSLPRSCA AAGTECLISG WGNTKSSGSS YPSLLQCLKA PVLSDSSCKS SYPGQITGNM ICVGFLEGGK DSCQGDSGGP VVCNGQLQGI VSWGYGCAQK NKPGVYTKVC NYVN WIQQ TIAAN . It is sometimes possible for the material contained within the vial of "Trypsin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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