Western Blot (WB)
(Western blot analysis of extracts of 293T cellline, using PIWIL2antibody|Immunohistochemistryof paraffin-embeddedhuman tonsil tissue usingPIWIL2antibody.)
PIWIL2 Antibody | anti-PIWIL2 antibody
PIWIL2 Antibody
IHC: 1:20-1:50
Western Blot (WB)
(Western blot analysis of extracts of 293T cellline, using PIWIL2antibody|Immunohistochemistryof paraffin-embeddedhuman tonsil tissue usingPIWIL2antibody.)
Western Blot (WB)
(Western blot analysis of extracts of 293T cellline, using PIWIL2antibody|Immunohistochemistryof paraffin-embeddedhuman tonsil tissue usingPIWIL2antibody.)
NCBI and Uniprot Product Information
NCBI Description
PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]
Uniprot Description
Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (). During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (). PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded (). Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (). Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (PubMed:16377660).
Research Articles on PIWIL2
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Product Notes
The PIWIL2 piwil2 (Catalog #AAA8516614) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The PIWIL2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PIWIL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:20-1:50. Researchers should empirically determine the suitability of the PIWIL2 piwil2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIWIL2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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