Rabbit anti-Human ESRRB Polyclonal Antibody | anti-ESRRB antibody

ESRRB Antibody

Cat. Num. AAA7107863

• Gene Names: ESRRB; ERR2; ERRb; ESRL2; NR3B2; DFNB35
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G purified
• Synonyms: ESRRB; Polyclonal Antibody; ESRRB Antibody; Steroid hormone receptor ERR2; ERR beta-2; Estrogen receptor-like 2; Estrogen-related receptor beta1; anti-ESRRB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 508

• Applicable Applications for anti-ESRRB antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:5000
• Immunogen: Recombinant Human Steroid hormone receptor ERR2 protein (432-502AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysateAll lanes: ESRRB antibody at 3.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49, 57, 56 KDaObserved band size: 49 KDa)

Related Product Information for anti-ESRRB antibody

Isoform 3: Transcription factor that binds a canonical ESRRB recognition (ERRE) sequence 5'TCAAGGTCA-3' localized on promoter and enhancer of targets genes regulating their expression or their transcription activity (PubMed:17920186, PubMed:19755138). Plays a role, in a LIF-independent manner, in maintainance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. Upon FGF signaling pathway activation, interacts with KDM1A by directly binding to enhancer site of ELF5 and EOMES and activating their transcription leading to self-renewal of trophoblast stem cells. Also regulates expression of multiple rod-specific genes and is required for survival of this cell type (By similarity). Plays a role as transcription factor activator of GATA6, NR0B1, POU5F1 and PERM1 (PubMed:23836911). Plays a role as transcription factor repressor of NFE2L2 transcriptional activity and ESR1 transcriptional activity (PubMed:17920186, PubMed:19755138). During mitosis remains bound to a subset of interphase target genes, including pluripotency regulators, through the canonical ESRRB recognition (ERRE) sequence, leading to their transcriptional activation in early G1 phase. Can coassemble on structured DNA elements with other transcription factors like SOX2, POU5F1, KDM1A and NCOA3 to trigger ESRRB-dependent gene activation. This mechanism, in the case of SOX2 corecruitment prevents the embryonic stem cells (ESCs) to epiblast stem cells (EpiSC) transition through positive regulation of NR0B1 that inhibits the EpiSC transcriptional program. Also plays a role inner ear development by controlling expression of ion channels and transporters and in early placentation (By similarity).

Product Categories/Family for anti-ESRRB antibody

Epigenetics and Nuclear Signaling; Cancer; Developmental biology; Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 238550159
• NCBI GeneID: 2103
• NCBI Accession #: NP_004443.3
• NCBI GenBank Nucleotide #: NM_004452.3
• UniProt Accession #: O95718; Q5F0P7; Q5F0P8; Q9HCB4; A2VDJ2; B6ZGU4
• Molecular Weight: 55,619 Da
• NCBI Official Full Name: steroid hormone receptor ERR2
• NCBI Official Synonym Full Names: estrogen related receptor beta
• NCBI Official Symbol: ESRRB
• NCBI Official Synonym Symbols: ERR2; ERRb; ESRL2; NR3B2; DFNB35
• NCBI Protein Information: steroid hormone receptor ERR2
• UniProt Protein Name: Steroid hormone receptor ERR2
• Protein Family: Steroid hormone receptor
• UniProt Gene Name: ESRRB
• UniProt Synonym Gene Names: ERR-beta

NCBI Description

This gene encodes a protein with similarity to the estrogen receptor. Its function is unknown; however, a similar protein in mouse plays an essential role in placental development. [provided by RefSeq, Jul 2008]

Uniprot Description

Isoform 3: Transcription factor that binds a canonical ESRRB recognition (ERRE) sequence 5'TCAAGGTCA-3' localized on promoter and enhancer of targets genes regulating their expression or their transcription activity (PubMed:17920186, PubMed:19755138). Plays a role, in a LIF-independent manner, in maintainance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. Upon FGF signaling pathway activation, interacts with KDM1A by directly binding to enhancer site of ELF5 and EOMES and activating their transcription leading to self-renewal of trophoblast stem cells. Also regulates expression of multiple rod-specific genes and is required for survival of this cell type (). Plays a role as transcription factor activator of GATA6, NR0B1, POU5F1 and PERM1 (PubMed:23836911). Plays a role as transcription factor repressor of NFE2L2 transcriptional activity and ESR1 transcriptional activity (PubMed:17920186, PubMed:19755138). During mitosis remains bound to a subset of interphase target genes, including pluripotency regulators, through the canonical ESRRB recognition (ERRE) sequence, leading to their transcriptional activation in early G1 phase. Can coassemble on structured DNA elements with other transcription factors like SOX2, POU5F1, KDM1A and NCOA3 to trigger ESRRB-dependent gene activation. This mechanism, in the case of SOX2 corecruitment prevents the embryonic stem cells (ESCs) to epiblast stem cells (EpiSC) transition through positive regulation of NR0B1 that inhibits the EpiSC transcriptional program. Also plays a role inner ear development by controlling expression of ion channels and transporters and in early placentation ().