Western Blot (WB)
(Western blot analysis of extract from starved NIH/3T3 cells, using MARCKS (Ab-158) antibody and MARCKS (phospho-Ser158) antibody.)
MARCKS Antibody | anti-MARCKS antibody
MARCKS (Ab-158) Antibody
Gene Names
MARCKS; MACS; 80K-L; PKCSL; PRKCSL
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
MARCKS; MARCKS (Ab-158) Antibody; anti-MARCKS antibody
Reactivity
Human, Mouse, Rat
Specificity
MARCKS (Ab-158) antibody detects endogenous levels of total MARCKS protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
332
Applicable Applications for anti-MARCKS antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-SP-F-K).
Preparation and Storage
Store at-20 degree C for 1 year
Western Blot (WB)
(Western blot analysis of extract from starved NIH/3T3 cells, using MARCKS (Ab-158) antibody and MARCKS (phospho-Ser158) antibody.)
Western Blot (WB)
(Western blot analysis of extract from starved NIH/3T3 cells, using MARCKS (Ab-158) antibody and MARCKS (phospho-Ser158) antibody.)
Product Categories/Family for anti-MARCKS antibody
References
Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412 Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609 Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,555 Da
NCBI Official Full Name
myristoylated alanine-rich C-kinase substrate
NCBI Official Synonym Full Names
myristoylated alanine rich protein kinase C substrate
NCBI Official Symbol
MARCKS
NCBI Official Synonym Symbols
MACS; 80K-L; PKCSL; PRKCSL
NCBI Protein Information
myristoylated alanine-rich C-kinase substrate
UniProt Protein Name
Myristoylated alanine-rich C-kinase substrate
Protein Family
UniProt Gene Name
MARCKS
UniProt Synonym Gene Names
MACS; PRKCSL; MARCKS; 80K-L protein; PKCSL
NCBI Description
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Research Articles on MARCKS
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Product Notes
The MARCKS marcks (Catalog #AAA856713) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The MARCKS (Ab-158) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MARCKS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the MARCKS marcks for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCKS, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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