Testing Data
(Adipogenesis inhibition of 3T3L1 cells: 3T3L1 cells were maintained in DMEM, w/10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1uM Dexamethasone, 0.5mM IBMX, 10ug/ml lnsulin (day 0) until day 2. The medium was replaced every 2 days w/new medium containing insulin in the presence or absence of 044747 (5ug/ml) and hCD137-Fc (Fc control). Staining w/Oil Red O was performed on day 7. Cells were washed twice w/PBS, fixed w/3.7% formalin, and stained w/0.5% filtered Oil Red O in propylene glycol.)
DLL1:Fc Active Protein
DLL1, Recombinant, Mouse:Fc (Delta-like Protein 1, Delta 1)
Purity
Highly Purified>90% (SDS-PAGE)
Synonyms
DLL1:Fc; DLL1; Recombinant; Mouse:Fc (Delta-like Protein 1; Delta 1); DLL1:Fc active protein
Host
Human, Mouse, HEK 293 cells
Specificity
Interacts with Notch receptors.
Purity/Purification
Highly Purified
>90% (SDS-PAGE)
>90% (SDS-PAGE)
Form/Format
Supplied as a Lyophilized powder from PBS, Reconstitute with 50ul sterile ddH2O
Sequence
Signal peptide and extracellular domain of mouse DLL1 (aa 1-545) is fused at the C-terminus to the Fc portion of human IgG1.
Biological Activity
Inhibits adipogenesis of 3T3L-1 cells. Induces Hes-1 in 3T3L-1 cells.
Endotoxin
<0.1 EU/ug (LAL)
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Adipogenesis inhibition of 3T3L1 cells: 3T3L1 cells were maintained in DMEM, w/10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1uM Dexamethasone, 0.5mM IBMX, 10ug/ml lnsulin (day 0) until day 2. The medium was replaced every 2 days w/new medium containing insulin in the presence or absence of 044747 (5ug/ml) and hCD137-Fc (Fc control). Staining w/Oil Red O was performed on day 7. Cells were washed twice w/PBS, fixed w/3.7% formalin, and stained w/0.5% filtered Oil Red O in propylene glycol.)
Testing Data
(Adipogenesis inhibition of 3T3L1 cells: 3T3L1 cells were maintained in DMEM, w/10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1uM Dexamethasone, 0.5mM IBMX, 10ug/ml lnsulin (day 0) until day 2. The medium was replaced every 2 days w/new medium containing insulin in the presence or absence of 044747 (5ug/ml) and hCD137-Fc (Fc control). Staining w/Oil Red O was performed on day 7. Cells were washed twice w/PBS, fixed w/3.7% formalin, and stained w/0.5% filtered Oil Red O in propylene glycol.)
Western Blot (WB)
(Induction of Hes-1 with the treatment of 044747: A mouse preadpipocyte cell line, 3T3L1, was stimulated with 044747 (5mg/ml) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.|Lane 1: 0min, Lane 2: 10min, Lane 3: 30min, Lane 4: 1hr, Lane 5: 2hr, Lane 6: 4hr, Lane 7: 8hr, Lane 8: 24hr.)
Western Blot (WB)
(Induction of Hes-1 with the treatment of 044747: A mouse preadpipocyte cell line, 3T3L1, was stimulated with 044747 (5mg/ml) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.|Lane 1: 0min, Lane 2: 10min, Lane 3: 30min, Lane 4: 1hr, Lane 5: 2hr, Lane 6: 4hr, Lane 7: 8hr, Lane 8: 24hr.)
Testing Data
(Adipogenesis inhibition. Macroscopic pictures.)
Testing Data
(Adipogenesis inhibition. Macroscopic pictures.)
Related Product Information for DLL1:Fc active protein
The Notch ligand delta-like protein 1 (DLL1) is essential for postnatal arteriogenesis and contributes to tumor progression. DLL1 is involved in differentiation and self-renewal of adipocyte stem cells. Blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK-cell precursor.
Product Categories/Family for DLL1:Fc active protein
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Product Notes
The DLL1:Fc (Catalog #AAA638035) is an Active Protein produced from Human, Mouse, HEK 293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Signal peptide and extracellu lar domain of mouse DLL1 (aa 1-545) is fused at the C-terminus to the Fc portion of human IgG1. It is sometimes possible for the material contained within the vial of "DLL1:Fc, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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