Human DLL1 ELISA Kit | DLL1 elisa kit

Human DLL1 PicoKine ELISA Kit

Cat. Num. AAA177309

• Gene Names: DLL1; DL1; Delta; DELTA1
• Reactivity: Human
• Synonyms: DLL1; Human DLL1 PicoKine ELISA Kit; Delta-like protein 1; delta-like 1 (Drosophila); Delta (Drosophila) like 1; Delta like 1 (Drosophila); Delta like 1; Delta like 1 protein; Delta like protein 1; Delta like protein 1 precursor; Delta; Drosophila; homolog of 1; Delta-like protein 1 [Precursor]; Delta1; DL1; DLL 1; Dll1; DLL1_HUMAN; Drosophila Delta homolog 1; H-Delta-1; OTTHUMP00000017690; DLL1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human DLL1
• Sequence Length: 723

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), urine and human milk
• Assay Type: Sandwich
• Detection Range: 78pg/ml-5000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human DLL1 PicoKine ELISA Kit standard curve )

Related Product Information for DLL1 elisa kit

Principle of the assay: human DLL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DLL1 has been precoated onto 96-well plates. Standards(NSO, S22-G540) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DLL1 amount of sample captured in plate.
Background: DLL1(DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

NCBI and Uniprot Product Information

• NCBI GI #: 118582508
• NCBI GeneID: 28514
• UniProt Accession #: O00548; Q9NU41; Q9UJV2; B2RAK7
• Molecular Weight: 78,056 Da
• NCBI Official Full Name: Delta-like protein 1
• NCBI Official Synonym Full Names: delta-like 1 (Drosophila)
• NCBI Official Symbol: DLL1
• NCBI Official Synonym Symbols: DL1; Delta; DELTA1
• NCBI Protein Information: delta-like protein 1; H-Delta-1; drosophila Delta homolog 1
• UniProt Protein Name: Delta-like protein 1
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL1
• UniProt Synonym Gene Names: Delta1; H-Delta-1
• UniProt Entry Name: DLL1_HUMAN

NCBI Description

DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL1: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Interacts with Notch receptors. Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; cytoplasmic vesicle

Molecular Function: protein binding; calcium ion binding; Notch binding

Biological Process: regulation of cell adhesion; Notch signaling pathway; negative regulation of myeloid cell differentiation; negative regulation of interleukin-10 production; Notch receptor processing; cell fate determination; compartment specification; somite specification; cell-cell signaling; negative regulation of auditory receptor cell differentiation; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; heart looping; determination of left/right symmetry; cell differentiation; inner ear development; positive regulation of Notch signaling pathway