Human Vitamin D Total ELISA Kit
Vitamin D (25OH) Total ELISA
Reactivity
Human
Synonyms
Vitamin D Total; Vitamin D (25OH) Total ELISA; Vitamin D Total elisa kit
Reactivity
Human
Specificity
Cross reactivity of the 25OH Vitamin D Total ELISA assay was determined by testing sera with spiked and unspiked cross reactants.
Samples
Serum
Assay Type
Sandwich
Preparation and Storage
2 - 8 degree C
Related Product Information for Vitamin D Total elisa kit
Intended Uses: Immunoenzymetnc assay for the measurement of human Calcitonin (CT) in serum. For research use only.
Principle of the Assay: The CT-U.S.-EASIA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1-human CT-MAb 2-HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic neaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colorimetrically by measuring the absorbance, which is proportional to the Calcitonin concentration. A calibration curve is plotted and Calcitonin concentration in samples is determined by interpolation from the calibration curve.
Principle of the Assay: The CT-U.S.-EASIA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1-human CT-MAb 2-HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic neaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colorimetrically by measuring the absorbance, which is proportional to the Calcitonin concentration. A calibration curve is plotted and Calcitonin concentration in samples is determined by interpolation from the calibration curve.
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Product Notes
The Human Vitamin D Total (Catalog #AAA495106-KAP1971) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495106-KAP1971 ELISA Kit recognizes Human Vitamin D Total. It is sometimes possible for the material contained within the vial of "Vitamin D Total, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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