VGF Nerve Growth Factor Inducible (VGF) Recombinant Protein | VGF recombinant protein

Recombinant VGF Nerve Growth Factor Inducible (VGF)

Cat. Num. AAA2125471

• Applications: SDS-Page, Western Blot
• Purity: Greater than 90% by SDS-PAGE
• Synonyms: VGF Nerve Growth Factor Inducible (VGF); Recombinant VGF Nerve Growth Factor Inducible (VGF); VGF/VGF Nerve Growth Factor Inducible; NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide; VGF recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% by SDS-PAGE
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Asp330~Pro449

• Applicable Applications for VGF recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Tag: N-terminal His and GST Tag
• Expression System: Prokaryotic expression
• Organism Species: Human
• Isoelectric Point: 3.8
• Subcellular Location: Secreted
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month, -80 degree C for 12 months; Stable for 12 months if correctly stored.; The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

Gene Sequencing

(Gene Sequencing (extract))

SDS-Page

(SDS-PAGE)

Product Categories/Family for VGF recombinant protein

Metabolic pathway; Infection immunity; Neuro science; Prokaryotic recombinant

NCBI and Uniprot Product Information

• NCBI GI #: 17136078
• NCBI GeneID: 7425
• NCBI Accession #: NP_003369.2
• NCBI GenBank Nucleotide #: NM_003378.3
• UniProt Accession #: O15240; Q9UDW8
• Molecular Weight: 67,258 Da
• NCBI Official Full Name: neurosecretory protein VGF
• NCBI Official Synonym Full Names: VGF nerve growth factor inducible
• NCBI Official Symbol: VGF
• NCBI Protein Information: neurosecretory protein VGF; neuro-endocrine specific protein VGF
• UniProt Protein Name: Neurosecretory protein VGF
• Protein Family: Neurosecretory protein
• UniProt Gene Name: VGF
• UniProt Synonym Gene Names: NERP-1; NERP-2
• UniProt Entry Name: VGF_HUMAN

NCBI Description

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle

Molecular Function: neuropeptide hormone activity; growth factor activity

Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; sexual reproduction; insulin secretion; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus