Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit | MAF elisa kit

Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit

Cat. Num. AAA2706434

• Gene Names: MAFG; hMAF
• Reactivity: Human
• Synonyms: V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF); Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit; c-MAF; Proto-oncogene c-Maf; Transcription factor Maf; MAF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF).; No significant cross-reactivity or interference between V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) and analogues was observed.
• Sequence Length: 162

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.074ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15147380
• NCBI GeneID: 4097
• Molecular Weight: 17,850 Da
• NCBI Official Full Name: V-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)
• NCBI Official Synonym Full Names: MAF bZIP transcription factor G
• NCBI Official Symbol: MAFG
• NCBI Official Synonym Symbols: hMAF
• NCBI Protein Information: transcription factor MafG
• UniProt Protein Name: Transcription factor MafG
• Protein Family: Maf-like protein
• UniProt Gene Name: MAFG

NCBI Description

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010]

Uniprot Description

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H+ ().