Rat V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit | JUN elisa kit

Rat V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit

Cat. Num. AAA2021040

• Gene Names: JUN; AP1; AP-1; c-Jun
• Reactivity: Rat
• Synonyms: V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN); Rat V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit; C-Jun; AP1; CJun; p39; Jun Oncogene; Proto-oncogene c-Jun; Activator protein 1; Transcription factor AP-1; JUN elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN).; No significant cross-reactivity or interference between V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) and analogues was observed.
• Sequence Length: 331

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JUN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for JUN elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119627033
• NCBI GeneID: 3725
• UniProt Accession #: P05412; Q6FHM7; Q96G93
• Molecular Weight: 35,676 Da
• NCBI Official Full Name: v-jun sarcoma virus 17 oncogene homolog (avian)
• NCBI Official Synonym Full Names: jun proto-oncogene
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: Viral jun-transforming protein
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_HUMAN

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Jun: the protooncogene c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The Jun Kinases (JNKs) binds to the N-terminal region of c-Jun and phosphorylate it in response to extracellular signals including growth factors, cytokines and stress, regulating its transcriptional activity.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: nucleoplasm; nuclear chromosome; transcription factor complex; transcriptional repressor complex; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; positive regulation of monocyte differentiation; response to cAMP; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; stress-activated MAPK cascade; microglial cell activation; response to lipopolysaccharide; SMAD protein nuclear translocation; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; negative regulation of cell proliferation; response to radiation; positive regulation of fibroblast proliferation; regulation of transcription factor activity; positive regulation of neuron apoptosis; transforming growth factor beta receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of neuron apoptosis; angiogenesis; toll-like receptor 4 signaling pathway; aging; response to drug; release of cytochrome c from mitochondria; monocyte differentiation; leading edge cell differentiation; MyD88-independent toll-like receptor signaling pathway; axon regeneration; learning; liver development; negative regulation of DNA binding; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; membrane depolarization; cellular response to potassium ion starvation; response to hydrogen peroxide; response to mechanical stimulus; response to cytokine stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication