Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) RTU ELISA Kit | MAF rtu elisa kit

Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit

Cat. Num. AAA4500886

• Gene Names: MAF; CCA4; AYGRP; c-MAF; CTRCT21
• Reactivity: Human
• Synonyms: V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF); Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit; c-MAF; Proto-oncogene c-Maf; Transcription factor Maf; MAF rtu elisa kit

• Reactivity: Human
• Sequence Length: 373

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.074ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAF rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 73427806
• NCBI GeneID: 4094
• NCBI Accession #: NP_001026974.1
• NCBI GenBank Nucleotide #: NM_001031804.2
• UniProt Accession #: O75444; Q66I47; Q9UP93
• Molecular Weight: 41,961 Da
• NCBI Official Full Name: transcription factor Maf isoform b
• NCBI Official Synonym Full Names: MAF bZIP transcription factor
• NCBI Official Symbol: MAF
• NCBI Official Synonym Symbols: CCA4; AYGRP; c-MAF; CTRCT21
• NCBI Protein Information: transcription factor Maf
• UniProt Protein Name: Transcription factor Maf
• Protein Family: Maf-like protein
• UniProt Gene Name: MAF

NCBI Description

The protein encoded by this gene is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in this gene are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (). When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.