Human Ubiquitin-Protein Ligase E3A (UBE3A) ELISA Kit | UBE3A elisa kit

Human Ubiquitin-Protein Ligase E3A (UBE3A) ELISA Kit

Cat. Num. AAA9713766

• Gene Names: UBE3A; AS; ANCR; E6-AP; HPVE6A; EPVE6AP
• Reactivity: Human
• Synonyms: Ubiquitin-Protein Ligase E3A (UBE3A); Human Ubiquitin-Protein Ligase E3A (UBE3A) ELISA Kit; UBE3A; ANCR; AS; E6-AP; EPVE6AP; FLJ26981; HPVE6A; CTCL tumor antigen se37-2; human papilloma virus E6-associated protein; oncogenic protein-associated protein E6-AP; UBE3A elisa kit

• Reactivity: Human
• Specificity: Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE3A. No significant cross-reactivity or interference between Human UBE3A and analogues was observed.
• Sequence Length: 875

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Ubiquitin-protein ligase E3A (UBE3A) Standard Curve.)

Related Product Information for UBE3A elisa kit

Description: This Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE3A in samples. An antibody specific for UBE3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBE3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Ubiquitin-protein ligase E3A (UBE3A) is an enzyme that in humans is encoded by the UBE3A gene. This enzyme is involved in targeting proteins for degradation within cells. Protein degradation is a normal process that removes damaged or unnecessary proteins and helps maintain the normal functions of cells. Ubiquitin protein ligase 3A attaches a small protein called ubiquitin to proteins that should be degraded. Cellular structures called proteasomes recognize and digest proteins tagged with ubiquitin. Both copies of the UBE3A gene are active in most of the body's tissues. In the brain, however, only the copy inherited from a person's mother (the maternal copy) is normally active. The UBE3A gene is located on the long (q) arm of chromosome 15 between positions 11 and 13, from base pair 23,133,488 to base pair 23,235,220.

NCBI and Uniprot Product Information

• NCBI GI #: 19718766
• NCBI GeneID: 7337
• NCBI Accession #: NP_000453.2
• NCBI GenBank Nucleotide #: NP_000453.2
• UniProt Accession #: Q05086
• NCBI Official Full Name: ubiquitin-protein ligase E3A isoform 2
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3A
• NCBI Official Symbol: UBE3A
• NCBI Official Synonym Symbols: AS; ANCR; E6-AP; HPVE6A; EPVE6AP
• NCBI Protein Information: ubiquitin-protein ligase E3A
• UniProt Protein Name: Ubiquitin-protein ligase E3A
• Protein Family: Ubiquitin-protein ligase
• UniProt Gene Name: UBE3A
• UniProt Synonym Gene Names: E6AP; EPVE6AP; HPVE6A
• UniProt Entry Name: UBE3A_HUMAN

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]