Mouse anti-Human UBE3A/E6-AP Monoclonal Antibody | anti-UBE3A antibody

UBE3A/E6-AP Mouse Monoclonal Antibody [Clone PCRP-UBE3A-1A2]

Cat. Num. AAA4382290

• Gene Names: UBE3A; AS; ANCR; E6-AP; HPVE6A; EPVE6AP; FLJ26981; UBE3A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: UBE3A/E6-AP; Monoclonal Antibody; UBE3A/E6-AP Mouse Monoclonal Antibody [Clone PCRP-UBE3A-1A2]; ANCR; Angelman syndrome (AS); CTCL tumor antigen se37-2; E6AP ubiquitin protein ligase; EPVE6AP; FLJ26981; HECT-type ubiquitin transferase E3A; Human papilloma virus E6 associated protein Angelman syndrome; HPVE6A; NY REN 54 antigen; Oncogenic protein associated protein E6AP; Renal carcinoma antigen NY REN 54;; anti-UBE3A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG1
• Clone Number: PCRP-UBE3A-1A2
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-UBE3A antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml
• Immunogen: Recombinant full-length human UBE3A protein
• Cellular Localization: Nucleus, Cytoplasm
• Positive Control: HeLa cells.
• Hu-chromosome Location: 15q11.2
• Unigene: 598862
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. UBE3A/E6-AP Mouse Monoclonal Antibody (PCRP-UBE3A-1A2) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )

Related Product Information for anti-UBE3A antibody

E6-associating protein (E6-AP), also designated ubiquitin protein ligase E3A(UBE3A), is a component of the ubiquitin-mediated proteolytic pathway thatselectively targets proteins for degradation by the 26S Proteasome. Ubiquitin(Ub) is directly conjugated to protein substrates by the transfer of Ub from anE2 ubiquitin conjugating enzyme to the target protein. This conjugation is facil-itated by the enzymatic activity of E3 ubiquitin ligase family members such asE6-AP. Several substrates of E6-AP have been identified and include the tumorsuppressor protein p53 and the mammalian homolog of Rad23, HHR23A. Previous studies have indicated that E6-AP associates with the humanpapillomavirus E6 oncogene, which forms a complex with p53 and therebypotentiates E6-AP mediated ubiquitination of p53. Genetic mutations thatimpair E6-AP activity result in the accumulation of p53 in the cytoplasm, andin many instances, these mutations are associated with the development ofthe rare neurodevelopmental disorder Angelman syndrome (AS), which ischaracterized by severe motor dysfunction and mental retardation.

NCBI and Uniprot Product Information

• NCBI GI #: 19718766
• NCBI GeneID: 7337
• UniProt Accession #: Q05086; P78355; Q93066; Q9UEP4; Q9UEP5; Q9UEP6; Q9UEP7; Q9UEP8; Q9UEP9; A8K8Z9
• NCBI Official Full Name: ubiquitin-protein ligase E3A isoform 2 [Homo sapiens]
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3A
• NCBI Official Symbol: UBE3A
• NCBI Official Synonym Symbols: AS; ANCR; E6-AP; HPVE6A; EPVE6AP; FLJ26981; UBE3A
• UniProt Protein Name: Ubiquitin-protein ligase E3A
• Protein Family: Ubiquitin-protein ligase
• UniProt Gene Name: UBE3A
• UniProt Synonym Gene Names: E6AP; EPVE6AP; HPVE6A
• UniProt Entry Name: UBE3A_HUMAN