Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit | TSG101 elisa kit

Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit

Cat. Num. AAA2023399

• Gene Names: TSG101; TSG10; VPS23
• Reactivity: Human
• Synonyms: Tumor Susceptibility Gene 101 (TSG101); Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit; TSG10; VPS23; Tumor Susceptibility Gene 10; ESCRT-I complex subunit TSG101; TSG101 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Susceptibility Gene 101 (TSG101).; No significant cross-reactivity or interference between Tumor Susceptibility Gene 101 (TSG101) and analogues was observed.
• Sequence Length: 285

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.114ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TSG101 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Susceptibility Gene 101 (TSG101). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Susceptibility Gene 101 (TSG101). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Susceptibility Gene 101 (TSG101), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Susceptibility Gene 101 (TSG101) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TSG101 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 5454140
• NCBI GeneID: 7251
• NCBI Accession #: NP_006283.1
• NCBI GenBank Nucleotide #: NM_006292.3
• UniProt Accession #: Q99816; Q9BUM5
• Molecular Weight: 31,732 Da
• NCBI Official Full Name: tumor susceptibility gene 101 protein
• NCBI Official Synonym Full Names: tumor susceptibility 101
• NCBI Official Symbol: TSG101
• NCBI Official Synonym Symbols: TSG10; VPS23
• NCBI Protein Information: tumor susceptibility gene 101 protein
• UniProt Protein Name: Tumor susceptibility gene 101 protein
• Protein Family: Tumor susceptibility gene 101 protein
• UniProt Gene Name: TSG101
• UniProt Entry Name: TS101_HUMAN

NCBI Description

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

Uniprot Description

TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: multivesicular body; late endosome membrane; early endosome; late endosome; cytoplasm; nucleolus; plasma membrane; endosome membrane

Molecular Function: ubiquitin binding; protein binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding

Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral reproduction; non-lytic virus budding; protein modification process; virus assembly; viral infectious cycle; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer