Human TSG101 (Tumor susceptibility gene 101 protein) ELISA Kit | TSG101 elisa kit

Human TSG101 (Tumor susceptibility gene 101 protein) ELISA Kit

Cat. Num. AAA7612204

• Gene Names: TSG101; TSG10; VPS23
• Reactivity: Human
• Synonyms: TSG101 (Tumor susceptibility gene 101 protein); Human TSG101 (Tumor susceptibility gene 101 protein) ELISA Kit; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101; TSG101 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TSG101. No significant cross-reactivity or interference between TSG101 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TSG101 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5454140
• NCBI GeneID: 7251
• NCBI Accession #: NP_006283.1
• NCBI GenBank Nucleotide #: NM_006292.3
• UniProt Accession #: Q99816; Q9BUM5
• Molecular Weight: 43,944 Da
• NCBI Official Full Name: tumor susceptibility gene 101 protein
• NCBI Official Synonym Full Names: tumor susceptibility gene 101
• NCBI Official Symbol: TSG101
• NCBI Official Synonym Symbols: TSG10; VPS23
• NCBI Protein Information: tumor susceptibility gene 101 protein; tumor susceptibility protein; ESCRT-I complex subunit TSG101
• UniProt Protein Name: Tumor susceptibility gene 101 protein
• Protein Family: Tumor susceptibility gene 101 protein
• UniProt Gene Name: TSG101
• UniProt Entry Name: TS101_HUMAN

NCBI Description

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

Uniprot Description

TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: multivesicular body; late endosome membrane; early endosome; cytoplasm; late endosome; plasma membrane; nucleolus; endosome membrane

Molecular Function: ubiquitin binding; protein binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding

Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; non-lytic virus budding; protein modification process; viral infectious cycle; virus assembly; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer