Mouse anti-Rat PCSK9 Monoclonal Antibody | anti-PCSK9 antibody

Anti-PCSK9 Antibody, Mouse Monoclonal

Cat. Num. AAA8112572

• Gene Names: Pcsk9; PC9; Narc1; NARC-1
• Reactivity: Rat
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: PCSK9; Monoclonal Antibody; Anti-PCSK9 Antibody; Mouse Monoclonal; PCSK9 Antibody; Mouse MAb; proprotein convertase subtilisin/kexin type 9; anti-PCSK9 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Clone Number: 04
• Specificity: Rat PCSK9.; No cross-reactivity in ELISA with Human PCSK9, Rhesus PCSK9
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PCSK9 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:50-1:200
• Immunogen: Recombinant Rat PCSK9 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat PCSK9 (rR PCSK9). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of rat PCSK9 in rat brain with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-PCSK9 antibody

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

Product Categories/Family for anti-PCSK9 antibody

Serine Proteases and Regulators

References

Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933. Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498 Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73. Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875. Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.

NCBI and Uniprot Product Information

• NCBI GI #: 77020250
• NCBI GeneID: 298296
• NCBI Accession #: NP_954862.2
• NCBI GenBank Nucleotide #: NM_199253.2
• UniProt Accession #: P59996; Q5I6U6
• Molecular Weight: 74,709 Da
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 9
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 9
• NCBI Official Symbol: Pcsk9
• NCBI Official Synonym Symbols: PC9; Narc1; NARC-1
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 9
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 9
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: Pcsk9
• UniProt Synonym Gene Names: Narc1; NARC-1; PC9

NCBI Description

a soluble zymogen; involved in hepatic growth and differentiation [RGD, Feb 2006]

Uniprot Description

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na+ channel (ENaC)-mediated Na+ absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways ().