Rabbit anti-Human UBE2H Polyclonal Antibody | anti-UBE2H antibody

UBE2H, CT (UBE2H, Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H) (FITC)

Cat. Num. AAA6360687

• Gene Names: UBE2H; GID3; UBC8; UBCH; UBCH2; E2-20K
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBE2H; Polyclonal Antibody; CT (UBE2H; Ubiquitin-conjugating enzyme E2 H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H) (FITC); Ubiquitin-protein ligase H; anti-UBE2H antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-UBE2H antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~183 amino acids from the C-terminal region of human UBE2H.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of UBE2H (arrow) using rabbit polyclonal UBE2H Antibody (S167). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2H gene.)

Product Categories/Family for anti-UBE2H antibody

Antibodies; Ubiquitin Ligases

References

Hayashida, S., et al., Genomics 66(2):221-225 (2000). Kaiser, P., et al., J. Biol. Chem. 269(12):8797-8802 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 33356154
• NCBI GeneID: 7328
• NCBI Accession #: NP_874356
• NCBI GenBank Nucleotide #: NM_182697.2
• UniProt Accession #: P62256; P37286; Q7Z6F4; A4D1L6; C9JY93
• Molecular Weight: 17,174 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 H isoform 2
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 H
• NCBI Official Symbol: UBE2H
• NCBI Official Synonym Symbols: GID3; UBC8; UBCH; UBCH2; E2-20K
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 H
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 H
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2H
• UniProt Entry Name: UBE2H_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]

Uniprot Description

UBE2H: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Mitochondrial; Ligase

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cytoplasm

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process