Typical Testing Data/Standard Curve (for reference only)
Human Trehalase (TREH) ELISA Kit | TREH elisa kit
Human Trehalase (TREH) ELISA Kit
Gene Names
TREH; TRE; TREA; TREHD
Reactivity
Human
Synonyms
Trehalase (TREH); Human Trehalase (TREH) ELISA Kit; TRE; TREA; Brush Border Membrane Glycoprotein; Alpha; Alpha-Trehalase; Alpha-Trehalose Glucohydrolase; TREH elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TREH. No significant cross-reactivity or interference between TREH and analogues was observed.
Sequence Length
552
Samples
Serum, plasma, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
<0.224ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TREH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TREH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TREH elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TREH in human serum, plasma, tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TREH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TREH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TREH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TREH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TREH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TREH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TREH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TREH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,941 Da
NCBI Official Full Name
trehalase isoform 2
NCBI Official Synonym Full Names
trehalase
NCBI Official Symbol
TREH
NCBI Official Synonym Symbols
TRE; TREA; TREHD
NCBI Protein Information
trehalase
UniProt Protein Name
Trehalase
UniProt Gene Name
TREH
UniProt Synonym Gene Names
TREA
NCBI Description
This gene encodes an enzyme that hydrolyses trehalose, a disaccharide formed from two glucose molecules found mainly in fungi, plants, and insects. A partial duplication of this gene is located adjacent to this locus on chromosome 11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Uniprot Description
Intestinal trehalase is probably involved in the hydrolysis of ingested trehalose.
Research Articles on TREH
Similar Products
Product Notes
The Human TREH treh (Catalog #AAA457211) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457211 ELISA Kit recognizes Human TREH. It is sometimes possible for the material contained within the vial of "Trehalase (TREH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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