Typical Testing Data/Standard Curve (for reference only)
Sheep Transient receptor potential cation channel, subfamily V, member 1 ELISA Kit | TRPV1 elisa kit
Sheep Transient receptor potential cation channel, subfamily V, member 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TRPV1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRPV1 antibody and an TRPV1-HRP conjugate. The assay sample and buffer are incubated together with TRPV1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRPV1 concentration since TRPV1 from samples and TRPV1-HRP conjugate compete for the anti-TRPV1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TRPV1 from the sample, fewer sites are left to bind TRPV1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRPV1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TRPV1: a receptor for capsaicin and a non-selective cation channel that is structurally related to members of the TRP family of ion channels. Activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.
Protein type: Receptor, misc.; Channel, cation; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17p13.2
Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; integral to membrane; cell junction; intrinsic to plasma membrane
Molecular Function: calmodulin binding; transmembrane receptor activity; excitatory extracellular ligand-gated ion channel activity; calcium channel activity; calcium-release channel activity; phosphoprotein binding; ATP binding
Biological Process: cell surface receptor linked signal transduction; transport; chemosensory behavior; thermoception; transmembrane transport
Research Articles on TRPV1
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Product Notes
The Sheep TRPV1 trpv1 (Catalog #AAA744880) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA744880 ELISA Kit recognizes Sheep TRPV1. It is sometimes possible for the material contained within the vial of "Transient receptor potential cation channel, subfamily V, member 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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