Human TRAF5 (TNF receptor associated factors 5) ELISA Kit | TRAF5 elisa kit

Human TRAF5 (TNF receptor associated factors 5)ELISA Kit

Cat. Num. AAA7608147

• Gene Names: TRAF5; RNF84; MGC:39780
• Reactivity: Human
• Synonyms: TRAF5 (TNF receptor associated factors 5); Human TRAF5 (TNF receptor associated factors 5)ELISA Kit; TNF receptor-associated factor 5; RING finger protein 84; TRAF5; RNF84; TRAF5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TRAF5. No significant cross-reactivity or interference between TRAF5 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRAF5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 30580600
• NCBI GeneID: 7188
• UniProt Accession #: O00463; Q6FHY1; B4DIS9; B4E0A2
• Molecular Weight: 557
• NCBI Official Full Name: TNF receptor-associated factor 5
• NCBI Official Synonym Full Names: TNF receptor-associated factor 5
• NCBI Official Symbol: TRAF5
• NCBI Official Synonym Symbols: RNF84; MGC:39780
• NCBI Protein Information: TNF receptor-associated factor 5; RING finger protein 84
• UniProt Protein Name: TNF receptor-associated factor 5
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF5
• UniProt Synonym Gene Names: RNF84
• UniProt Entry Name: TRAF5_HUMAN

NCBI Description

The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAF5: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Belongs to the TNF receptor-associated factor family. A subfamily.

Protein type: Adaptor/scaffold; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: centrosome; internal side of plasma membrane; cytoplasm; cytosol

Molecular Function: protein binding; signal transducer activity; zinc ion binding; thioesterase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; positive regulation of cell proliferation; protein ubiquitination; positive regulation of transcription factor activity; signal transduction; activation of NF-kappaB transcription factor