TNF receptor-associated factor 5 (TRAF5) Recombinant Protein | TRAF5 recombinant protein

Recombinant Human TNF receptor-associated factor 5 (TRAF5)

Cat. Num. AAA1014127

• Gene Names: TRAF5; RNF84; MGC:39780
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: TNF receptor-associated factor 5 (TRAF5); Recombinant Human TNF receptor-associated factor 5 (TRAF5); RING finger protein 84; TRAF5 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-557. Full Length
• Sequence: MAYSEEHKGMPCGFIRQNSGNSISLDFEPSIEYQFVERLEERYKCAFCHSVLHNPHQTGCGHRFCQHCILSLRELNTVPICPVDKEVIKSQEVFKDNCCKREVLNLYVYCSNAPGCNAKVILGRYQDHLQQCLFQPVQCSNEKCREPVLRKDLKEHLSASCQFRKEKCLYCKKDVVVINLQNHEENLCPEYPVFCPNNCAKIILKTEVDEHLAVCPEAEQDCPFKHYGCAVTDKRRNLQQHEHSALREHMRLVLEKNVQLEEQISDLHKSLEQKESKIQQLAETIKKLEKEFKQFAQLFGKNGSFLPNIQVFASHIDKSAWLEAQVHQLLQMVNQQQNKFDLRPLMEAVDTVKQKITLLENNDQRLAVLEEETNKHDTHINIHKAQLSKNEERFKLLEGTCYNGKLIWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQSGKKNIMETFKPDPNSSSFKRPDGEMNIASGCPRFVAHSVLENAKNAYIKDDTLFLKVAVDLTDLEDL

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for TRAF5 recombinant protein

Adapter protein and signal transducer that links mbers of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Ses to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.

Product Categories/Family for TRAF5 recombinant protein

Immunology

References

Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5) .Mizushima S., Fujita M., Ishida T., Azuma S., Kato K., Hirai M., Otsuka M., Yamamoto T., Inoue J.Gene 207:135-140(1998) Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004) Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Human TNF receptor-associated factor 5 (TRAF5) cDNA cloning, expression and assignment of the TRAF5 gene to chromosome 1q32.Nakano H., Shindo M., Yamada K., Yoshida M.C., Santee S.M., Ware C.F., Jenkins N.A., Gilbert D.J., Yagita H., Copeland N.G., Okumura K.Genomics 42:26-32(1997) The TNF-receptor-associated factor family scaffold molecules for cytokine receptors, kinases and their regulators.Wajant H., Henkler F., Scheurich P.Cell. Signal. 13:389-400(2001) Tumor necrosis factor receptor-associated factors (TRAFs) .Bradley J.R., Pober J.S.Oncogene 20:6482-6491(2001) Herpesvirus entry mediator, a member of the tumor necrosis factor receptor (TNFR) family, interacts with members of the TNFR-associated factor family and activates the transcription factors NF-kappaB and AP-1.Marsters S.A., Ayres T.M., Skubatch M., Gray C.L., Rothe M., Ashkenazi A.J. Biol. Chem. 272:14029-14032(1997) ATAR, a novel tumor necrosis factor receptor family member, signals through TRAF2 and TRAF5.Hsu H., Solovyev I., Colombero A., Elliott R., Kelley M., Boyle W.J.J. Biol. Chem. 272:13471-13474(1997) Tumor necrosis factor (TNF) -mediated kinase cascades bifurcation of nuclear factor-kappaB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2.Song H.Y., Regnier C.H., Kirschning C.J., Goeddel D.V., Rothe M.Proc. Natl. Acad. Sci. U.S.A. 94:9792-9796(1997) Tumor necrosis factor receptor-associated factor (TRAF) 5 and TRAF2 are involved in CD30-mediated NFkappaB activation.Aizawa S., Nakano H., Ishida T., Horie R., Nagai M., Ito K., Yagita H., Okumura K., Inoue J., Watanabe T.J. Biol. Chem. 272:2042-2045(1997) ASK1 is essential for JNK/SAPK activation by TRAF2.Nishitoh H., Saitoh M., Mochida Y., Takeda K., Nakano H., Rothe M., Miyazono K., Ichijo H.Mol. Cell 2:389-395(1998) CD40-tumor necrosis factor receptor-associated factor (TRAF) interactions regulation of CD40 signaling through multiple TRAF binding sites and TRAF hetero-oligomerization.Pullen S.S., Miller H.G., Everdeen D.S., Dang T.T., Crute J.J., Kehry M.R.Biochemistry 37:11836-11845(1998) Activation of OX40 signal transduction pathways leads to tumor necrosis factor receptor-associated factor (TRAF) 2- and TRAF5-mediated NF-kappaB activation.Kawamata S., Hori T., Imura A., Takaori-Kondo A., Uchiyama T.J. Biol. Chem. 273:5808-5814(1998) RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase.McCarthy J.V., Ni J., Dixit V.M.J. Biol. Chem. 273:16968-16975(1998) TAJ, a novel member of the tumor necrosis factor receptor family, activates the c-Jun N-terminal kinase pathway and mediates caspase-independent cell death.Eby M.T., Jasmin A., Kumar A., Sharma K., Chaudhary P.M.J. Biol. Chem. 275:15336-15342(2000) The TRAF family of signal transducers mediates NF-kappaB activation by the TRANCE receptor.Wong B.R., Josien R., Lee S.Y., Vologodskaia M., Steinman R.M., Choi Y.J. Biol. Chem. 273:28355-28359(1998) TTRAP, a novel protein that associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor-associated factors (TRAFs) , and that inhibits nuclear factor-kappa B activation.Pype S., Declercq W., Ibrahimi A., Michiels C., Van Rietschoten J.G.I., Dewulf N., de Boer M., Vandenabeele P., Huylebroeck D., Remacle J.E.J. Biol. Chem. 275:18586-18593(2000) TACI is a TRAF-interacting receptor for TALL-1, a tumor necrosis factor family member involved in B cell regulation.Xia X.-Z., Treanor J., Senaldi G., Khare S.D., Boone T., Kelley M., Theill L.E., Colombero A., Solovyev I., Lee F., McCabe S., Elliott R., Miner K., Hawkins N., Guo J., Stolina M., Yu G., Wang J., Delaney J., Meng S.-Y., Boyle W.J., Hsu H.J. Exp. Med. 192:137-143(2000) B cell maturation protein is a receptor for the tumor necrosis factor family member TALL-1.Shu H.-B., Johnson H.Proc. Natl. Acad. Sci. U.S.A. 97:9156-9161(2000) TRAF family proteins link PKR with NF-kappa B activation.Gil J., Garcia M.A., Gomez-Puertas P., Guerra S., Rullas J., Nakano H., Alcami J., Esteban M.Mol. Cell. Biol. 24:4502-4512(2004) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.Sci. Signal. 2:RA46-RA46(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 77404348
• NCBI GeneID: 7188
• NCBI Accession #: NP_001029082.1
• NCBI GenBank Nucleotide #: NM_001033910.2
• UniProt Accession #: O00463; Q6FHY1; B4DIS9; B4E0A2
• Molecular Weight: 80.4 kDa
• NCBI Official Full Name: TNF receptor-associated factor 5 isoform b
• NCBI Official Synonym Full Names: TNF receptor associated factor 5
• NCBI Official Symbol: TRAF5
• NCBI Official Synonym Symbols: RNF84; MGC:39780
• NCBI Protein Information: TNF receptor-associated factor 5
• UniProt Protein Name: TNF receptor-associated factor 5
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF5
• UniProt Synonym Gene Names: RNF84
• UniProt Entry Name: TRAF5_HUMAN

NCBI Description

The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

TRAF5: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Belongs to the TNF receptor-associated factor family. A subfamily.

Protein type: Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: centrosome; cytoplasm; cytosol; internal side of plasma membrane

Molecular Function: protein binding; signal transducer activity; thioesterase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; apoptosis; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription factor activity; protein ubiquitination; regulation of apoptosis; signal transduction