Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tomoregulin-2 (TMEFF2) Standard Curve.)
Human Tomoregulin-2 (TMEFF2) ELISA Kit | TMEFF2 elisa kit
Human Tomoregulin-2 (TMEFF2) ELISA Kit
Gene Names
TMEFF2; TR; HPP1; TPEF; TR-2; TENB2; CT120.2
Reactivity
Human
Synonyms
Tomoregulin-2 (TMEFF2); Human Tomoregulin-2 (TMEFF2) ELISA Kit; TMEFF2; HPP1; TENB2; TPEF; TR; tomoregulin; transmembrane protein TENB2; TMEFF2 elisa kit
Reactivity
Human
Specificity
Human Tomoregulin-2 (TMEFF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEFF2. No significant cross-reactivity or interference between Human TMEFF2 and analogues was observed.
Sequence Length
175
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tomoregulin-2 (TMEFF2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tomoregulin-2 (TMEFF2) Standard Curve.)
Related Product Information for TMEFF2 elisa kit
Description: This Human Tomoregulin-2 (TMEFF2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEFF2 in samples. An antibody specific for TMEFF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEFF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEFF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEFF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 368-amino acid TRa protein contains 2 follistatin-like domains and a conserved EGF-like domain. It also has a predicted N-terminal signal peptide, a transmembrane domain, a cytosolic tail G protein-activating motif, and potential sites for N-linked glycosylation and glycosaminoglycan attachment. Unlike other EGF/NRG family members, TRa has a histidine replacing a conserved arginine essential for EGF receptor recognition. The TRb and TRc proteins contain 418 and 379 amino acids, respectively, and differ from one another and TRa in the cytoplasmic domain. Northern blot analysis detected expression of approximately 2.2- and 3.1-kb TR transcripts predominantly in brain. The 374-amino acid TMEFF2 protein shares approximately 36% and 99% sequence identity with TMEFF1 and mouse Tmeff2, respectively.
Background: The deduced 368-amino acid TRa protein contains 2 follistatin-like domains and a conserved EGF-like domain. It also has a predicted N-terminal signal peptide, a transmembrane domain, a cytosolic tail G protein-activating motif, and potential sites for N-linked glycosylation and glycosaminoglycan attachment. Unlike other EGF/NRG family members, TRa has a histidine replacing a conserved arginine essential for EGF receptor recognition. The TRb and TRc proteins contain 418 and 379 amino acids, respectively, and differ from one another and TRa in the cytoplasmic domain. Northern blot analysis detected expression of approximately 2.2- and 3.1-kb TR transcripts predominantly in brain. The 374-amino acid TMEFF2 protein shares approximately 36% and 99% sequence identity with TMEFF1 and mouse Tmeff2, respectively.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tomoregulin-2 isoform 3
NCBI Official Synonym Full Names
transmembrane protein with EGF like and two follistatin like domains 2
NCBI Official Symbol
TMEFF2
NCBI Official Synonym Symbols
TR; HPP1; TPEF; TR-2; TENB2; CT120.2
NCBI Protein Information
tomoregulin-2
UniProt Protein Name
Tomoregulin-2
Protein Family
UniProt Gene Name
TMEFF2
UniProt Synonym Gene Names
HPP1; TENB2; TPEF; TR-2
NCBI Description
This gene encodes a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Uniprot Description
May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
Research Articles on TMEFF2
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Product Notes
The Human TMEFF2 tmeff2 (Catalog #AAA9713965) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713965 ELISA Kit recognizes Human TMEFF2. It is sometimes possible for the material contained within the vial of "Tomoregulin-2 (TMEFF2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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