Mouse anti-Human LYST Monoclonal Antibody | anti-LYST antibody

LYST (Lysosomal-trafficking Regulator, Beige Homolog, CHS, CHS1) (HRP)

Cat. Num. AAA6153380

• Gene Names: LYST; CHS; CHS1
• Reactivity: Human
• Applications: ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LYST; Monoclonal Antibody; LYST (Lysosomal-trafficking Regulator; Beige Homolog; CHS; CHS1) (HRP); anti-LYST antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3H1
• Specificity: Recognizes human LYST.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-LYST antibody: ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1-100 from human LYST with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSTDSNSLAREFLTDVNRLCNAVVQRVEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEEKATDFNLPL
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged LYST is 0.03ng/ml as a capture antibody)

Related Product Information for anti-LYST antibody

May be required for sorting endosomal resident proteins into late multivesicular endosomes by a mechanism involving microtubules.

Product Categories/Family for anti-LYST antibody

Antibodies; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 54292123
• NCBI GeneID: 1130
• NCBI Accession #: NP_000072.2
• NCBI GenBank Nucleotide #: NM_000081
• UniProt Accession #: Q99698; O43274; Q5T2U9; Q96TD7; Q96TD8; Q99709; Q9H133
• Molecular Weight: 429,139 Da
• NCBI Official Full Name: lysosomal-trafficking regulator
• NCBI Official Synonym Full Names: lysosomal trafficking regulator
• NCBI Official Symbol: LYST
• NCBI Official Synonym Symbols: CHS; CHS1
• NCBI Protein Information: lysosomal-trafficking regulator; beige homolog; Chediak-Higashi syndrome 1
• UniProt Protein Name: Lysosomal-trafficking regulator
• Protein Family: Lysosomal-trafficking regulator
• UniProt Gene Name: LYST
• UniProt Synonym Gene Names: CHS; CHS1
• UniProt Entry Name: LYST_HUMAN

NCBI Description

This gene encodes a protein that regulates intracellular protein trafficking in endosomes, and may be involved in pigmentation. Mutations in this gene are associated with Chediak-Higashi syndrome, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants, though the full-length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2013]

Uniprot Description

LYST: May be required for sorting endosomal resident proteins into late multivesicular endosomes by a mechanism involving microtubules. Defects in LYST are the cause of Chediak-Higashi syndrome (CHS). CHS is a rare autosomal recessive disorder characterized by hypopigmentation, severe immunologic deficiency, a bleeding tendency, neurologic abnormalities, abnormal intracellular transport to and from the lysosome, and giant inclusion bodies in a variety of cell types. Most patients die at an early age unless they receive an allogeneic hematopoietic stem cell transplant (SCT). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q42.1-q42.2

Cellular Component: microtubule cytoskeleton; extrinsic to membrane; endomembrane system; cytosol

Molecular Function: protein binding; phospholipid binding

Biological Process: response to drug; secretion of lysosomal enzymes; natural killer cell mediated cytotoxicity; T cell mediated immunity; leukocyte chemotaxis; neutrophil mediated immunity; mast cell secretory granule organization and biogenesis; defense response to protozoan; endosome to lysosome transport via multivesicular body sorting pathway; positive regulation of natural killer cell activation; microtubule-based process; pigmentation; melanosome organization and biogenesis; lysosome organization and biogenesis; phospholipid metabolic process; defense response to bacterium; blood coagulation; defense response to virus; phospholipid homeostasis

Disease: Chediak-higashi Syndrome