Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tolloid-like protein 2 (TLL2) Standard Curve.)
Human Tolloid-Like Protein 2 (TLL2) ELISA Kit | TLL2 elisa kit
Human Tolloid-Like Protein 2 (TLL2) ELISA Kit
Reactivity
Human
Synonyms
Tolloid-Like Protein 2 (TLL2); Human Tolloid-Like Protein 2 (TLL2) ELISA Kit; TLL2; KIAA0932; MGC133312; MGC133313; TLL2 elisa kit
Reactivity
Human
Specificity
Human Tolloid-like protein 2 (TLL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLL2. No significant cross-reactivity or interference between Human TLL2 and analogues was observed.
Sequence Length
1015
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tolloid-like protein 2 (TLL2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tolloid-like protein 2 (TLL2) Standard Curve.)
Related Product Information for TLL2 elisa kit
Description: This Human Tolloid-like protein 2 (TLL2) ELISA Kit employs a two-site sandwich ELISA to quantitate TLL2 in samples. An antibody specific for TLL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TLL2 encodes an astacin-like zinc-dependent metalloprotease and is a subfamily member of the metzincin family. Unlike other family members, a similar protein in mice does not cleave procollagen C-propeptides or chordin.TLL2 shares 80.3% amino acid sequence identity with BMP1, including 870 overlapping residues. Analysis by RT-PCR ELISA demonstrated weak expression of TLL2 in most tissues and moderate expression in heart and brain.TLL2 encodes a deduced 1,015-amino acid protein with a molecular mass of 120 kD by SDS/PAGE.Whereas BMP1, TLD, and TLL1 specifically process procollagen C-propeptides at the physiologically relevant site, TLL2 lacks this activity. BMP1 and TLL1 cleave chordin, but TLL2 and TLD do not.
Background: TLL2 encodes an astacin-like zinc-dependent metalloprotease and is a subfamily member of the metzincin family. Unlike other family members, a similar protein in mice does not cleave procollagen C-propeptides or chordin.TLL2 shares 80.3% amino acid sequence identity with BMP1, including 870 overlapping residues. Analysis by RT-PCR ELISA demonstrated weak expression of TLL2 in most tissues and moderate expression in heart and brain.TLL2 encodes a deduced 1,015-amino acid protein with a molecular mass of 120 kD by SDS/PAGE.Whereas BMP1, TLD, and TLL1 specifically process procollagen C-propeptides at the physiologically relevant site, TLL2 lacks this activity. BMP1 and TLL1 cleave chordin, but TLL2 and TLD do not.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tolloid-like protein 2
NCBI Official Synonym Full Names
tolloid like 2
NCBI Official Symbol
TLL2
NCBI Protein Information
tolloid-like protein 2
UniProt Protein Name
Tolloid-like protein 2
Protein Family
UniProt Gene Name
TLL2
UniProt Synonym Gene Names
KIAA0932
UniProt Entry Name
TLL2_HUMAN
NCBI Description
This gene encodes an astacin-like zinc-dependent metalloprotease and is a subfamily member of the metzincin family. Unlike other family members, a similar protein in mice does not cleave procollagen C-propeptides or chordin. [provided by RefSeq, Jul 2008]
Uniprot Description
TLL2: Protease which specifically processes pro-lysyl oxidase. Required for the embryonic development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis. Belongs to the peptidase M12A family.
Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.24.-
Chromosomal Location of Human Ortholog: 10q23-q24
Cellular Component: extracellular region
Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding
Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; multicellular organismal development; proteolysis; cell differentiation
Research Articles on TLL2
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Product Notes
The Human TLL2 tll2 (Catalog #AAA9713986) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713986 ELISA Kit recognizes Human TLL2. It is sometimes possible for the material contained within the vial of "Tolloid-Like Protein 2 (TLL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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