Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tolloid-like protein 1 (TLL1) Standard Curve.)
Mouse Tolloid-Like Protein 1 (TLL1) ELISA Kit | TLL1 elisa kit
Mouse Tolloid-Like Protein 1 (TLL1) ELISA Kit
Gene Names
Tll1; Tll; Tll-1; b2b2476Clo
Reactivity
Mouse
Synonyms
Tolloid-Like Protein 1 (TLL1); Mouse Tolloid-Like Protein 1 (TLL1) ELISA Kit; TLL1; ASD6; TLL; TLL1 elisa kit
Reactivity
Mouse
Specificity
Mouse Tolloid-like protein 1 (TLL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TLL1. No significant cross-reactivity or interference between Mouse TLL1 and analogues was observed.
Sequence Length
1013
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tolloid-like protein 1 (TLL1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tolloid-like protein 1 (TLL1) Standard Curve.)
Related Product Information for TLL1 elisa kit
Description: This Mouse Tolloid-like protein 1 (TLL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TLL1 in samples. An antibody specific for TLL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TLL1 encodes an astacin-like zinc-dependent metalloprotease and is a subfamily member of the metzincin family. A similar protein in mice is required during heart development and specifically processes procollagen C-propeptides and chordin at similar cleavage sites. As previously demonstrated for BMP1 and TLD, TLL1 specifically processes procollagen C-propeptides at the physiologically relevant site, whereas TLL2 lacks this activity. BMP1 and TLL1 cleave chordin, at sites similar to procollagen C-propeptide cleavage sites, and counteract dorsalizing effects of chordin upon overexpression on Xenopus embryos. Proteases TLD and TLL2 do not cleave chordin.TLL1 gene plays multiple roles in formation of the mammalian heart and is essential for formation of the interventricular septum.
Background: TLL1 encodes an astacin-like zinc-dependent metalloprotease and is a subfamily member of the metzincin family. A similar protein in mice is required during heart development and specifically processes procollagen C-propeptides and chordin at similar cleavage sites. As previously demonstrated for BMP1 and TLD, TLL1 specifically processes procollagen C-propeptides at the physiologically relevant site, whereas TLL2 lacks this activity. BMP1 and TLL1 cleave chordin, at sites similar to procollagen C-propeptide cleavage sites, and counteract dorsalizing effects of chordin upon overexpression on Xenopus embryos. Proteases TLD and TLL2 do not cleave chordin.TLL1 gene plays multiple roles in formation of the mammalian heart and is essential for formation of the interventricular septum.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tolloid-like protein 1
NCBI Official Synonym Full Names
tolloid-like
NCBI Official Symbol
Tll1
NCBI Official Synonym Symbols
Tll; Tll-1; b2b2476Clo
NCBI Protein Information
tolloid-like protein 1
UniProt Protein Name
Tolloid-like protein 1
Protein Family
UniProt Gene Name
Tll1
UniProt Synonym Gene Names
Tll; mTll
UniProt Entry Name
TLL1_MOUSE
Research Articles on TLL1
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Product Notes
The Mouse TLL1 tll1 (Catalog #AAA9716824) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716824 ELISA Kit recognizes Mouse TLL1. It is sometimes possible for the material contained within the vial of "Tolloid-Like Protein 1 (TLL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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