Rabbit anti-Human, Mouse PSMD8 Polyclonal Antibody | anti-PSMD8 antibody

PSMD8 Polyclonal Antibody

Cat. Num. AAA3011864

• Gene Names: PSMD8; S14; p31; HIP6; HYPF; Nin1p; Rpn12; HEL-S-91n
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PSMD8; Polyclonal Antibody; PSMD8 Polyclonal Antibody; 26S proteasome non-ATPase regulatory subunit 8; 26S proteasome regulatory subunit RPN12; 26S proteasome regulatory subunit S14; p31; anti-PSMD8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PSMD8 polyclonal antibody detects endogenous levels of PSMD8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 350

• Applicable Applications for anti-PSMD8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human PSMD8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using PSMD8 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using PSMD8 antibody.)

Related Product Information for anti-PSMD8 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.

NCBI and Uniprot Product Information

• NCBI GI #: 308153477
• NCBI GeneID: 5714
• UniProt Accession #: P48556
• Molecular Weight: ~ 30-35kDa
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 8
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 8
• NCBI Official Symbol: PSMD8
• NCBI Official Synonym Symbols: S14; p31; HIP6; HYPF; Nin1p; Rpn12; HEL-S-91n
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 8
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 8
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD8

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.